Experimental data

Lipidomics studies on human plasma

• NIDDK / NIST human plasma sample analysis

Lipidomics studies on macrophages

• RAW 264.7 cells
  • Kdo₂-Lipid A time course experiments
  • Compactin/Kdo₂-Lipid A time course experiments

• Primary macrophages
  • Thioglycolate-elicited peritoneal macrophages (TGEM)
    • Compactin/Kdo₂-Lipid A time course experiments
    • Foam cell study with wild-type and LDLR(-/-) mice
  • Bone marrow-derived macrophages (BMDM)
    • Kdo₂-Lipid A time course experiments
    • ATP/Kdo₂-Lipid A time course experiments
  • Compare results from TGEM and BMDM treated with Kdo₂-Lipid A (3 time points: 0,6,24 hrs)
• Compare results from RAW, TGEM, and BMDM for sterols treated with Kdo₂-Lipid A (full time course: 8 time points)

Microarray analysis of macrophages

• RAW 264.7 cells
  • Kdo₂-Lipid A time course experiments
  • Compactin/Kdo₂-Lipid A time course experiments (2 time points: 12, 24hrs)
  • Compactin/Kdo₂-Lipid A time course experiments (full time course: 7 time points)

• Primary macrophages
  • Thioglycolate-elicited macrophages
    • Foam cell study with LDLR(-/-) and wild-type mice
    • Kdo₂-Lipid A time course experiments
    • Compactin/Kdo₂-Lipid A time course experiments
  • Bone marrow-derived macrophages
    • Compactin/Kdo₂-Lipid A time course experiments
    • ATP/Kdo₂-Lipid A time course experiments

Subcellular fractionation studies on macrophages

• RAW 264.7 cells
  • Kdo₂-Lipid A experiments