Microarray data for Sc5d: fold-change ratios

Bone marrow-derived macrophage cells treated with Kdo2-Lipid A (KLA) and ATP

Gene Time course graph Description Treatment 15min 30min 1h 2h 4h 8h 20h
Sc5d
KLA  ATP  ATP+KLA
 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830073K23 product:unclassifiable, full insert sequence [AK080673] KLA .62 .56 .53 .46 .38 .29 .35
 ATP .87 .91 1.37 .86 .56 .43 .33
 ATP+KLA .54 .49 .72 .72 .72 .39 .20
Sc5d
KLA  ATP  ATP+KLA
 sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) (Sc5d), mRNA [NM_172769] KLA 1.18 1.19 1.17 1.09 .95 .63 .70
 ATP .99 1.00 1.31 1.09 .84 1.04 1.51
 ATP+KLA 1.09 1.08 1.30 1.20 1.05 .88 .76

Bone marrow-derived macrophage cells treated with 25-hydroxy-cholesterol +/- lipid-depleted media containing compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Sc5d
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830073K23 product:unclassifiable, full insert sequence [AK080673] 25OHC/Ctl (?) .93 .77 .57 .43 .40 .40 .36
 25-OHC*/* (?) .91 .69 .45 .22 .19 .21 .20
 
 
Sc5d
25OHC/Ctl - treatment with 25-OHC / no treatment
25-OHC*/* - lipid depleted media containing compactin and treatment with 25-OHC / lipid depleted media containing compactin only
sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) (Sc5d), mRNA [NM_172769] 25OHC/Ctl (?) .96 .97 .72 .50 .40 .37 .37
 25-OHC*/* (?) .93 1.00 .63 .29 .14 .14 .18
 
 

Thioglycolate-elicited macrophage cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Sc5d
KLA  Compactin  Compactin+KLA
 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830073K23 product:unclassifiable, full insert sequence [AK080673] KLA .93 .71 .52 .41 .26 .26 .29
 Compactin 1.01 .93 1.02 1.00 .98 1.06 1.03
 Compactin+KLA 1.04 .78 .53 .41 .28 .28 .31
Sc5d
KLA  Compactin  Compactin+KLA
 sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) (Sc5d), mRNA [NM_172769] KLA 1.06 .92 .97 .85 .62 .42 .49
 Compactin 1.00 .91 1.09 1.04 1.10 1.08 1.08
 Compactin+KLA .99 .95 1.11 .99 .76 .50 .54

RAW 264.7 cells treated with Kdo2-Lipid A (KLA) and compactin

Gene Time course graph Description Treatment 30min 1h 2h 4h 8h 12h 24h
Sc5d
KLA  Compactin  Compactin+KLA
 10 days neonate cortex cDNA, RIKEN full-length enriched library, clone:A830073K23 product:unclassifiable, full insert sequence [AK080673] KLA 1.21 1.09 .67 .62 .58 .64 .62
 Compactin 1.03 1.00 1.23 1.15 1.03 .89 .92
 Compactin+KLA 1.23 1.02 .83 .68 .55 .59 .60
Sc5d
KLA  Compactin  Compactin+KLA
 sterol-C5-desaturase (fungal ERG3, delta-5-desaturase) homolog (S. cerevisae) (Sc5d), mRNA [NM_172769] KLA 1.04 1.20 1.17 .97 1.00 1.16 1.63
 Compactin .97 1.06 1.03 1.13 .92 .99 1.10
 Compactin+KLA .97 1.15 1.30 1.12 .98 1.16 1.58