Gene/Proteome Database (LMPD)

LMPD ID
LMP000869
Gene ID
84002
Species
Homo sapiens (Human)
Gene Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Gene Symbol
B3GNT5
Synonyms
B3GN-T5; beta3Gn-T5
Alternate Names
lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase; BGnT-5; lc3 synthase; beta-1,3-Gn-T5; lc(3)Cer synthase; lactotriaosylceramide synthase; beta-1,3-N-acetylglucosaminyltransferase 5; beta-1,3-N-acetylglucosaminyltransferase bGnT-5; beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase; UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
Chromosome
3
Map Location
3q28
EC Number
2.4.1.206
Summary
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for B3GNT5

Proteins

lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
Refseq ID NP_114436
Protein GI 14042986
UniProt ID Q9BYG0
mRNA ID NM_032047
Length 378
RefSeq Status REVIEWED
MRMLVSGRRVKKWQLIIQLFATCFLASLMFFWEPIDNHIVSHMKSYSYRYLINSYDFVNDTLSLKHTSAGPRYQYLINHKEKCQAQDVLLLLFVKTAPENYDRRSGIRRTWGNENYVRSQLNANIKTLFALGTPNPLEGEELQRKLAWEDQRYNDIIQQDFVDSFYNLTLKLLMQFSWANTYCPHAKFLMTADDDIFIHMPNLIEYLQSLEQIGVQDFWIGRVHRGAPPIRDKSSKYYVSYEMYQWPAYPDYTAGAAYVISGDVAAKVYEASQTLNSSLYIDDVFMGLCANKIGIVPQDHVFFSGEGKTPYHPCIYEKMMTSHGHLEDLQDLWKNATDPKVKTISKGFFGQIYCRLMKIILLCKISYVDTYPCRAAFI

Gene Information

Entrez Gene ID
84002
Gene Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Gene Symbol
B3GNT5
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0000139 TAS:Reactome C Golgi membrane
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005622 IDA:UniProtKB C intracellular
GO:0008457 IDA:UniProtKB F beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity
GO:0008378 IEA:InterPro F galactosyltransferase activity
GO:0047256 IEA:UniProtKB-EC F lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity
GO:0008917 IEA:Ensembl F lipopolysaccharide N-acetylglucosaminyltransferase activity
GO:0016266 TAS:Reactome P O-glycan processing
GO:0044267 TAS:Reactome P cellular protein metabolic process
GO:0007417 IDA:UniProtKB P central nervous system development
GO:0009247 TAS:UniProtKB P glycolipid biosynthetic process
GO:0043687 TAS:Reactome P post-translational protein modification
GO:0006486 TAS:UniProtKB P protein glycosylation

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_200727 O-linked glycosylation
REACT_115606 O-linked glycosylation of mucins

Domain Information

InterPro Annotations

Accession Description
IPR002659 Glycosyl transferase, family 31

UniProt Annotations

Entry Information

Gene Name
UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
Protein Entry
B3GN5_HUMAN
UniProt ID
Q9BYG0
Species
Human

Comments

Comment Type Description
Catalytic Activity UDP-N-acetyl-D-glucosamine + beta-D- galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N- acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D- glucosyl-(1<->1)-ceramide.
Function Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development.
Induction Up-regulated by stimulation with retinoic acid and down-regulated with 12-O-tetradecanoylphorbol-13-acetate (TPA).
Pathway Protein modification; protein glycosylation.
Sequence Caution Sequence=BAD92754.1; Type=Frameshift; Positions=347; Evidence= ;
Similarity Belongs to the glycosyltransferase 31 family.
Subcellular Location Golgi apparatus membrane ; Single-pass type II membrane protein .
Tissue Specificity Widely expressed. Highly expressed in lung, colon, placenta, testis, pituitary gland and cerebellum. Weakly expressed in brain, liver, spleen, lymph node and thymus.
Web Resource Name=Functional Glycomics Gateway - GTase; Note=Beta1,3-N-acetylglucosaminyltransferase 5; URL="http://www.functionalglycomics.org/glycomics/molecule/jsp/glycoEnzyme/viewGlycoEnzyme.jsp?gbpId=gt_hum_538";
Web Resource Name=GGDB; Note=GlycoGene database; URL="http://jcggdb.jp/rcmg/ggdb/Homolog?cat=symbol&symbol=B3GNT5";

Identical and Related Proteins

Unique RefSeq proteins for LMP000869 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
14042986 RefSeq NP_114436 378 lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase

Identical Sequences to LMP000869 proteins

Reference Database Accession Length Protein Name
GI:14042986 GenBank AHD72459.1 378 Sequence 9527 from patent US 8586006
GI:14042986 GenBank AIC52503.1 378 B3GNT5, partial [synthetic construct]
GI:14042986 RefSeq XP_005247881.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X2 [Homo sapiens]
GI:14042986 RefSeq XP_005247882.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X3 [Homo sapiens]
GI:14042986 RefSeq XP_005247883.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X4 [Homo sapiens]
GI:14042986 RefSeq XP_005247884.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase isoform X5 [Homo sapiens]

Related Sequences to LMP000869 proteins

Reference Database Accession Length Protein Name
GI:14042986 DBBJ BAG52370.1 378 unnamed protein product [Homo sapiens]
GI:14042986 EMBL CAC83093.1 376 Gal-beta1-3 GlcNAc-transferase [Homo sapiens]
GI:14042986 GenBank ABA66861.1 378 Sequence 2503 from patent US 6943241
GI:14042986 RefSeq XP_002814384.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase [Pongo abelii]
GI:14042986 RefSeq XP_003832032.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase [Pan paniscus]
GI:14042986 RefSeq XP_008955613.1 378 PREDICTED: lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase [Pan paniscus]