Gene/Proteome Database (LMPD)

LMPD ID
LMP002007
Gene ID
8529
Species
Homo sapiens (Human)
Gene Name
cytochrome P450, family 4, subfamily F, polypeptide 2
Gene Symbol
CYP4F2
Synonyms
CPF2
Alternate Names
phylloquinone omega-hydroxylase CYP4F2; CYPIVF2; 20-HETE synthase; cytochrome P450 4F2; cytochrome P450-LTB-omega; arachidonic acid omega-hydroxylase; leukotriene-B(4) 20-monooxygenase 1; leukotriene-B(4) omega-hydroxylase 1; 20-hydroxyeicosatetraenoic acid synthase; cytochrome P450, subfamily IVF, polypeptide 2
Chromosome
19
Map Location
19p13.12
EC Number
1.14.13.194
Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away. [provided by RefSeq, Jul 2008]
Orthologs
View orthologs and multiple alignments for CYP4F2

Proteins

phylloquinone omega-hydroxylase CYP4F2 precursor
Refseq ID NP_001073
Protein GI 13435391
UniProt ID P78329
mRNA ID NM_001082
Length 520
RefSeq Status REVIEWED
MSQLSLSWLGLWPVAASPWLLLLLVGASWLLAHVLAWTYAFYDNCRRLRCFPQPPRRNWFWGHQGMVNPTEEGMRVLTQLVATYPQGFKVWMGPISPLLSLCHPDIIRSVINASAAIAPKDKFFYSFLEPWLGDGLLLSAGDKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSACLDMFEHISLMTLDSLQKCVFSFDSHCQEKPSEYIAAILELSALVSKRHHEILLHIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPSQGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAHLPFLTMCMKESLRLHPPVPVISRHVTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPENIKERSPLAFIPFSAGPRNCIGQTFAMAEMKVVLALTLLRFRVLPDHTEPRRKPELVLRAEGGLWLRVEPLS
sig_peptide: 1..16 inference: COORDINATES: ab initio prediction:SignalP:4.0 calculated_mol_wt: 1759 peptide sequence: MSQLSLSWLGLWPVAA mat_peptide: 17..520 product: phylloquinone omega-hydroxylase CYP4F2 calculated_mol_wt: 58113 peptide sequence: SPWLLLLLVGASWLLAHVLAWTYAFYDNCRRLRCFPQPPRRNWFWGHQGMVNPTEEGMRVLTQLVATYPQGFKVWMGPISPLLSLCHPDIIRSVINASAAIAPKDKFFYSFLEPWLGDGLLLSAGDKWSRHRRMLTPAFHFNILKPYMKIFNESVNIMHAKWQLLASEGSACLDMFEHISLMTLDSLQKCVFSFDSHCQEKPSEYIAAILELSALVSKRHHEILLHIDFLYYLTPDGQRFRRACRLVHDFTDAVIQERRRTLPSQGVDDFLQAKAKSKTLDFIDVLLLSKDEDGKKLSDEDIRAEADTFMFEGHDTTASGLSWVLYHLAKHPEYQERCRQEVQELLKDREPKEIEWDDLAHLPFLTMCMKESLRLHPPVPVISRHVTQDIVLPDGRVIPKGIICLISVFGTHHNPAVWPDPEVYDPFRFDPENIKERSPLAFIPFSAGPRNCIGQTFAMAEMKVVLALTLLRFRVLPDHTEPRRKPELVLRAEGGLWLRVEPLS

Gene Information

Entrez Gene ID
8529
Gene Name
cytochrome P450, family 4, subfamily F, polypeptide 2
Gene Symbol
CYP4F2
Species
Homo sapiens

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0016324 IDA:UniProtKB C apical plasma membrane
GO:0005737 IDA:UniProtKB C cytoplasm
GO:0005789 TAS:UniProtKB C endoplasmic reticulum membrane
GO:0043231 IDA:UniProtKB C intracellular membrane-bounded organelle
GO:0018685 IDA:BHF-UCL F alkane 1-monooxygenase activity
GO:0052871 IDA:UniProtKB F alpha-tocopherol omega-hydroxylase activity
GO:0008392 IDA:UniProtKB F arachidonic acid epoxygenase activity
GO:0052869 IDA:UniProtKB F arachidonic acid omega-hydroxylase activity
GO:0020037 IEA:InterPro F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0050051 IDA:UniProtKB F leukotriene-B4 20-monooxygenase activity
GO:0016709 IDA:UniProtKB F oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
GO:0052872 IDA:UniProtKB F tocotrienol omega-hydroxylase activity
GO:0047057 IMP:UniProtKB F vitamin-K-epoxide reductase (warfarin-sensitive) activity
GO:0019369 IDA:UniProtKB P arachidonic acid metabolic process
GO:0007596 TAS:UniProtKB P blood coagulation
GO:0017144 IMP:UniProtKB P drug metabolic process
GO:0019373 IDA:UniProtKB P epoxygenase P450 pathway
GO:0006690 TAS:Reactome P icosanoid metabolic process
GO:0036101 IDA:UniProtKB P leukotriene B4 catabolic process
GO:0006691 TAS:Reactome P leukotriene metabolic process
GO:0001676 IDA:BHF-UCL P long-chain fatty acid metabolic process
GO:0042361 IDA:UniProtKB P menaquinone catabolic process
GO:0030195 IMP:UniProtKB P negative regulation of blood coagulation
GO:0032304 IMP:UniProtKB P negative regulation of icosanoid secretion
GO:0097267 TAS:Reactome P omega-hydroxylase P450 pathway
GO:0055114 IDA:UniProtKB P oxidation-reduction process
GO:0042376 IDA:UniProtKB P phylloquinone catabolic process
GO:0032305 IMP:UniProtKB P positive regulation of icosanoid secretion
GO:0003095 IEP:UniProtKB P pressure natriuresis
GO:0008217 IMP:UniProtKB P regulation of blood pressure
GO:0003091 IEP:UniProtKB P renal water homeostasis
GO:0044281 TAS:Reactome P small molecule metabolic process
GO:0055078 IEP:UniProtKB P sodium ion homeostasis
GO:0000038 IDA:BHF-UCL P very long-chain fatty acid metabolic process
GO:0042360 IDA:UniProtKB P vitamin E metabolic process
GO:0042371 IMP:UniProtKB P vitamin K biosynthetic process
GO:0042377 IDA:UniProtKB P vitamin K catabolic process
GO:0006805 TAS:Reactome P xenobiotic metabolic process

REACTOME Pathway Links

REACTOME Pathway ID Description
REACT_13425 Miscellaneous substrates
REACT_150134 Synthesis of (16-20)-hydroxyeicosatetraenoic acids (HETE)
REACT_150420 Synthesis of Leukotrienes (LT) and Eoxins (EX)

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002401 Cytochrome P450, E-class, group I
IPR017972 Cytochrome P450, conserved site

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 4, subfamily F, polypeptide 2
Protein Entry
CP4F2_HUMAN
UniProt ID
P78329
Species
Human

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=P78329-1; Sequence=Displayed; Name=2; IsoId=P78329-2; Sequence=VSP_055578, VSP_055579, VSP_055580; Note=No experimental confirmation available.;
Biophysicochemical Properties Kinetic parameters: KM=74.8 uM for leukotriene-B(4) ; KM=1.7 uM for menaquinone-4 (MK-4) ; Vmax=2.42 nmol/min/mg enzyme ; Note=kcat is 0.067 min(-1) with menaquinone-4 (MK-4) as substrate ;
Catalytic Activity (5Z,8Z,11Z,14Z)-icosatetraenoate + NADPH + O(2) = (5Z,8Z,11Z,14Z)-20-hydroxyicosa-5,8,11,14-tetraenoate + NADP(+) + H(2)O.
Catalytic Activity (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa- 6,8,10,14-tetraenoate + NADPH + O(2) = (6Z,8E,10E,14Z)-(5S,12R)- 5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + NADP(+) + H(2)O.
Catalytic Activity Phylloquinone + NADPH + O(2) = omega- hydroxyphylloquinone + NADP(+) + H(2)O.
Cofactor Name=heme; Xref=ChEBI
Disease Coumarin resistance (CMRES) [MIM
Function Omega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B(4) omega-hydroxylase by mediating conversion of leukotriene-B(4) (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene- B(4) (20-OH LTB4) (PubMed:8026587, PubMed:9799565). {ECO
Interaction P16333:NCK1; NbExp=2; IntAct=EBI-1752413, EBI-389883;
Pathway Cofactor degradation; phylloquinone degradation.
Similarity Belongs to the cytochrome P450 family.
Subcellular Location Microsome membrane {ECO
Tissue Specificity Liver. Also present in kidney: specifically expressed in the S2 and S3 segments of proximal tubules in cortex and outer medulla (PubMed:10660572). {ECO
Web Resource Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/cyp4f2/";

Identical and Related Proteins

Unique RefSeq proteins for LMP002007 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13435391 RefSeq NP_001073 520 phylloquinone omega-hydroxylase CYP4F2 precursor

Identical Sequences to LMP002007 proteins

Reference Database Accession Length Protein Name
GI:13435391 GenBank AAL67578.1 520 cytochrome P450, subfamily IVF, polypeptide 2 [Homo sapiens]
GI:13435391 GenBank AAH67439.1 520 Cytochrome P450, family 4, subfamily F, polypeptide 2 [Homo sapiens]
GI:13435391 GenBank EAW84509.1 520 cytochrome P450, family 4, subfamily F, polypeptide 2, isoform CRA_b [Homo sapiens]
GI:13435391 GenBank EAW84510.1 520 cytochrome P450, family 4, subfamily F, polypeptide 2, isoform CRA_b [Homo sapiens]
GI:13435391 GenBank AFO05742.1 520 Sequence 380 from patent US 8216786
GI:13435391 SwissProt P78329.1 520 RecName: Full=Phylloquinone omega-hydroxylase CYP4F2; AltName: Full=20-hydroxyeicosatetraenoic acid synthase; Short=20-HETE synthase; AltName: Full=Arachidonic acid omega-hydroxylase; AltName: Full=CYPIVF2; AltName: Full=Cytochrome P450 4F2; AltName: Full=Cytochrome P450-LTB-omega; AltName: Full=Leukotriene-B(4) 20-monooxygenase 1; AltName: Full=Leukotriene-B(4) omega-hydroxylase 1; Flags: Precursor [Homo sapiens]

Related Sequences to LMP002007 proteins

Reference Database Accession Length Protein Name
GI:13435391 DBBJ BAA75823.1 520 Leukotriene B4 omega-hydroxylase [Homo sapiens]
GI:13435391 DBBJ BAF83479.1 520 unnamed protein product [Homo sapiens]
GI:13435391 GenBank AAH67440.1 520 Cytochrome P450, family 4, subfamily F, polypeptide 2 [Homo sapiens]
GI:13435391 GenBank AAH67437.1 520 Cytochrome P450, family 4, subfamily F, polypeptide 2 [Homo sapiens]
GI:13435391 GenBank AIC55423.1 520 CYP4F2, partial [synthetic construct]
GI:13435391 RefSeq XP_003813618.1 520 PREDICTED: leukotriene-B(4) omega-hydroxylase 1 isoform X1 [Pan paniscus]