Gene/Proteome Database (LMPD)

LMPD ID
LMP002250
Gene ID
74147
Species
Mus musculus (Mouse)
Gene Name
enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Gene Symbol
Ehhadh
Synonyms
1300002P22Rik; HD; L-PBE; LBFP; LBP; MFP; MFP1; PBFE
Alternate Names
peroxisomal bifunctional enzyme; PBE; L-bifunctional enzyme; L-specific multifunctional beta-oxdiation protein
Chromosome
16
Map Location
16 B1|16
EC Number
4.2.1.17

Proteins

peroxisomal bifunctional enzyme
Refseq ID NP_076226
Protein GI 31541815
UniProt ID Q9DBM2
mRNA ID NM_023737
Length 718
RefSeq Status VALIDATED
MAEYLRLPHSLAMIRLCNPPVNAISPTVITEVRNGLQKASLDHTVRAIVICGANDNFCAGADIHGFKSPTGLTLGSLVDEIQRYQKPVVAAIQGVALGGGLELALGCHYRIANAKARVGFPEVMLGILPGARGTQLLPRVVGVPVALDLITSGRHISTDEALKLGILDVVVKSDPVEEAIKFAQTVIGKPIEPRRILNKPVPSLPNMDSVFAEAIAKVRKQYPGRLAPETCVRSVQASVKHPYEVAIKEEAKLFMYLRGSGQARALQYAFFAEKSANKWSTPSGASWKTASAQPVSSVGVLGLGTMGRGIAISFARVGIPVVAVESDPKQLDTAKKIITSTLEKEASKSGQASAKPNLRFSSSTKELSSVDLVIEAVFEDMNLKKKVFAELSALCKPGAFLCTNTSALDVDDIASSTDRPQLVIGTHFFSPAHIMRLLEVIPSRYSSPTTIATVMSLSKRIGKIGVVVGNCYGFVGNRMLAPYYNQGYFLIEEGSKPEDVDGVLEEFGFRMGPFRVSDLAGLDVGWKVRKGQGLTGPSLPPGTPTRKRGNTRYSPIADMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLSEFLSQYRETHHIKQRSISKEEILERCLYSLINEAFRILEEGMAASPEHIDVIYLHGYGWPRHVGGPMYYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYLRRLVAQGSPPLKEWQSLAGPHSSKL

Gene Information

Entrez Gene ID
74147
Gene Name
enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Gene Symbol
Ehhadh
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IEA:Ensembl C cytosol
GO:0005739 IDA:MGI C mitochondrion
GO:0005634 IEA:Ensembl C nucleus
GO:0005777 TAS:MGI C peroxisome
GO:0003857 TAS:MGI F 3-hydroxyacyl-CoA dehydrogenase activity
GO:0050662 IEA:InterPro F coenzyme binding
GO:0004165 IEA:UniProtKB-EC F dodecenoyl-CoA delta-isomerase activity
GO:0004300 IEA:UniProtKB-EC F enoyl-CoA hydratase activity
GO:0006637 TAS:MGI P acyl-CoA metabolic process
GO:0006635 IMP:MGI P fatty acid beta-oxidation
GO:0006475 ISS:UniProtKB P internal protein amino acid acetylation

KEGG Pathway Links

KEGG Pathway ID Description
mmu01200 Carbon metabolism
mmu03320 PPAR signaling pathway
mmu04146 Peroxisome

Domain Information

InterPro Annotations

Accession Description
IPR006108 3-hydroxyacyl-CoA dehydrogenase, C-terminal
IPR006176 3-hydroxyacyl-CoA dehydrogenase, NAD binding
IPR006180 3-hydroxyacyl-CoA dehydrogenase, conserved site
IPR008927 6-phosphogluconate dehydrogenase, C-terminal-like
IPR029045 ClpP/crotonase-like domain
IPR001753 Crotonase_core_superfam
IPR013328 Dehydrogenase, multihelical
IPR018376 Enoyl-CoA hydratase/isomerase, conserved site
IPR016040 NAD(P)-binding domain

UniProt Annotations

Entry Information

Gene Name
enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Protein Entry
ECHP_MOUSE
UniProt ID
Q9DBM2
Species
Mouse

Comments

Comment Type Description
Catalytic Activity (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O.
Catalytic Activity (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl- CoA.
Catalytic Activity (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH.
Enzyme Regulation Enzyme activity enhanced by acetylation. {ECO:0000250}.
Pathway Lipid metabolism; fatty acid beta-oxidation.
Ptm Acetylated, leading to enhanced enzyme activity. Acetylation is enhanced by up to 80% after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with highest increase on Lys-344. Acetylation and enzyme activity increased by about 1.5% on addition of fatty acids (By similarity). {ECO:0000250}.
Similarity In the C-terminal section; belongs to the 3- hydroxyacyl-CoA dehydrogenase family. {ECO:0000305}.
Similarity In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}.
Subcellular Location Peroxisome {ECO:0000250}.
Subunit Monomer. {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP002250 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
31541815 RefSeq NP_076226 718 peroxisomal bifunctional enzyme

Identical Sequences to LMP002250 proteins

Reference Database Accession Length Protein Name
GI:31541815 GenBank AAH16899.1 718 Enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [Mus musculus]
GI:31541815 GenBank EDK97607.1 718 enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [Mus musculus]
GI:31541815 SwissProt Q9DBM2.4 718 RecName: Full=Peroxisomal bifunctional enzyme; Short=PBE; Short=PBFE; Includes: RecName: Full=Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; Includes: RecName: Full=3-hydroxyacyl-CoA dehydrogenase [Mus musculus]

Related Sequences to LMP002250 proteins

Reference Database Accession Length Protein Name
GI:31541815 DBBJ BAB23628.1 718 unnamed protein product [Mus musculus]
GI:31541815 EMBL CBV18079.1 722 unnamed protein product [Rattus norvegicus]
GI:31541815 GenBank AAA41825.1 722 peroxisomal enoyl-CoA: hydrotase-3-hydroxyacyl-CoA [Rattus norvegicus]
GI:31541815 GenBank AAH89777.1 722 Enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase [Rattus norvegicus]
GI:31541815 GenBank AGX95964.1 722 Sequence 72188 from patent US 8541208
GI:31541815 SwissProt P07896.2 722 RecName: Full=Peroxisomal bifunctional enzyme; Short=PBE; Short=PBFE; Includes: RecName: Full=Enoyl-CoA hydratase/3,2-trans-enoyl-CoA isomerase; Includes: RecName: Full=3-hydroxyacyl-CoA dehydrogenase [Rattus norvegicus]