Gene/Proteome Database (LMPD)

LMPD ID
LMP004100
Gene ID
13124
Species
Mus musculus (Mouse)
Gene Name
cytochrome P450, family 8, subfamily b, polypeptide 1
Gene Symbol
Cyp8b1
Synonyms
-
Alternate Names
7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; CYPVIIIB1; cytochrome P450 8B1; sterol 12-alpha-hydrolase; sterol 12-alpha-hydroxylase; cytochrome P450, 8b1, sterol 12 alpha-hydrolase; 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase
Chromosome
9
Map Location
9 F4|9 72.73 cM
EC Number
1.14.13.95

Proteins

7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
Refseq ID NP_034142
Protein GI 227497652
UniProt ID O88962
mRNA ID NM_010012
Length 500
RefSeq Status VALIDATED
MTLWCTVLGALLTVVGCLCLSLLLRHRRPWEPPLDKGFVPWLGHSMAFRKNMFEFLKGMRAKHGDVFTVQLGGQYFTFVMDPLSFGPIIKNTEKALDFQSYAKELVLKVFGYQSVDGDHRMIHLASTKHLMGQGLEELNQAMLDSLSLVMLGPKGSSLGASSWCEDGLFHFCYRILFKAGFLSLFGYTKDKQQDLDEADELFRKFRRFDFLFPRFVYSLLGPREWVEVSQLQRLFHQRLSVKQNLEKDGISCWLGYMLQFLREQGIASSMQDKFNFMMLWASQGNTGPTCFWVLLFLLKHQDAMKAVREEATRVMGKARLEAKKSFTFTPSALKHTPVLDSVMEESLRLCATPTLLRVVQEDYVLKMASGQEYQIRRGDKVALFPYLSVHMDPDIHPEPTAFKYDRFLNPDGTRKVDFYKSGKKIHHYSMPWGSGVSKCPGRFFALSEMKTFVLLMIMYFDFKLVDPDIPVPPIDPRRWGFGTSQPSHEVRFLYRLKPVQ

Gene Information

Entrez Gene ID
13124
Gene Name
cytochrome P450, family 8, subfamily b, polypeptide 1
Gene Symbol
Cyp8b1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IBA:RefGenome C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0033778 IEA:UniProtKB-EC F 7alpha-hydroxycholest-4-en-3-one 12alpha-hydroxylase activity
GO:0020037 IBA:RefGenome F heme binding
GO:0005506 IEA:InterPro F iron ion binding
GO:0008397 IBA:RefGenome F sterol 12-alpha-hydroxylase activity
GO:0006629 IBA:RefGenome P lipid metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
mmu03320 PPAR signaling pathway
mmu00120 Primary bile acid biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001128 Cytochrome P450
IPR002403 Cytochrome P450, E-class, group IV
IPR024204 Cytochrome P450, cholesterol 7-alpha-monooxygenase-type

UniProt Annotations

Entry Information

Gene Name
cytochrome P450, family 8, subfamily b, polypeptide 1
Protein Entry
CP8B1_MOUSE
UniProt ID
O88962
Species
Mouse

Comments

Comment Type Description
Catalytic Activity 7-alpha-hydroxycholest-4-en-3-one + NADPH + O(2) = 7-alpha,12-alpha-dihydroxycholest-4-en-3-one + NADP(+) + H(2)O.
Cofactor Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
Function Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha- hydroxylated C27 steroids.
Similarity Belongs to the cytochrome P450 family. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane; Single-pass membrane protein. Microsome membrane; Single-pass membrane protein.
Tissue Specificity Expressed in liver.

Identical and Related Proteins

Unique RefSeq proteins for LMP004100 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
227497652 RefSeq NP_034142 500 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase

Identical Sequences to LMP004100 proteins

Reference Database Accession Length Protein Name
GI:227497652 GenBank AAD19876.1 500 sterol 12-alpha-hydroxylase CYP8B1 [Mus musculus]

Related Sequences to LMP004100 proteins

Reference Database Accession Length Protein Name
GI:227497652 GenBank AAC63036.1 500 sterol 12-alpha hydroxylase CYP8B1 [Mus musculus]
GI:227497652 GenBank AAH10973.1 500 Cytochrome P450, family 8, subfamily b, polypeptide 1 [Mus musculus]
GI:227497652 GenBank AAH10974.1 500 Cytochrome P450, family 8, subfamily b, polypeptide 1 [Mus musculus]
GI:227497652 GenBank AAH49969.1 500 Cytochrome P450, family 8, subfamily b, polypeptide 1 [Mus musculus]
GI:227497652 GenBank EDL09122.1 500 cytochrome P450, family 8, subfamily b, polypeptide 1 [Mus musculus]
GI:227497652 SwissProt O88962.1 500 RecName: Full=7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase; AltName: Full=7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase; AltName: Full=CYPVIIIB1; AltName: Full=Cytochrome P450 8B1; AltName: Full=Sterol 12-alpha-hydroxylase [Mus musculus]