Gene/Proteome Database (LMPD)

LMPD ID
LMP005596
Gene ID
59035
Species
Mus musculus (Mouse)
Gene Name
coactivator-associated arginine methyltransferase 1
Gene Symbol
Carm1
Synonyms
Prmt4
Alternate Names
histone-arginine methyltransferase CARM1; protein arginine methyltransferase; protein arginine N-methyltransferase 4
Chromosome
9
Map Location
9 A4|9
EC Number
2.1.1.-

Proteins

histone-arginine methyltransferase CARM1 isoform 1
Refseq ID NP_067506
Protein GI 50511310
UniProt ID Q9WVG6
mRNA ID NM_021531
Length 608
RefSeq Status VALIDATED
MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGIALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLANTGIVNHTHSRMGSIMSTGIVQGSSGAQGGGGSSSAHYAVNNQFTMGGPAISMASPMSIPTNTMHYGS
histone-arginine methyltransferase CARM1 isoform 2
Refseq ID NP_694781
Protein GI 189409145
UniProt ID Q9WVG6
mRNA ID NM_153141
Length 585
RefSeq Status VALIDATED
MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGIALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSERTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARKIYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYLKPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILMAKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVRCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHYTSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLGSSGAQGGGGSSSAHYAVNNQFTMGGPAISMASPMSIPTNTMHYGS

Gene Information

Entrez Gene ID
59035
Gene Name
coactivator-associated arginine methyltransferase 1
Gene Symbol
Carm1
Species
Mus musculus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0005634 IDA:UniProtKB C nucleus
GO:0042054 IDA:UniProtKB F histone methyltransferase activity
GO:0035642 IDA:UniProtKB F histone methyltransferase activity (H3-R17 specific)
GO:0030374 IDA:UniProtKB F ligand-dependent nuclear receptor transcription coactivator activity
GO:0070577 IDA:UniProtKB F lysine-acetylated histone binding
GO:0042803 IPI:UniProtKB F protein homodimerization activity
GO:0008276 IDA:MGI F protein methyltransferase activity
GO:0016274 IDA:UniProtKB F protein-arginine N-methyltransferase activity
GO:0035242 IDA:UniProtKB F protein-arginine omega-N asymmetric methyltransferase activity
GO:0003713 IDA:UniProtKB F transcription coactivator activity
GO:0044212 IMP:UniProtKB F transcription regulatory region DNA binding
GO:0060350 IMP:MGI P endochondral bone morphogenesis
GO:0034971 IDA:UniProtKB P histone H3-R17 methylation
GO:0034970 IEA:Ensembl P histone H3-R2 methylation
GO:0034969 IDA:UniProtKB P histone arginine methylation
GO:0016571 IDA:MGI P histone methylation
GO:0030520 IGI:MGI P intracellular estrogen receptor signaling pathway
GO:0030518 IDA:HGNC P intracellular steroid hormone receptor signaling pathway
GO:0032091 IDA:MGI P negative regulation of protein binding
GO:0009405 IEA:InterPro P pathogenesis
GO:0035246 IDA:GOC P peptidyl-arginine N-methylation
GO:0019919 IDA:GOC P peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
GO:0008284 IMP:MGI P positive regulation of cell proliferation
GO:0045600 IMP:UniProtKB P positive regulation of fat cell differentiation
GO:0006479 IMP:MGI P protein methylation
GO:0003420 IMP:MGI P regulation of growth plate cartilage chondrocyte proliferation
GO:0033146 IDA:UniProtKB P regulation of intracellular estrogen receptor signaling pathway
GO:0006355 IDA:MGI P regulation of transcription, DNA-templated
GO:0051591 IEA:Ensembl P response to cAMP
GO:0006351 IEA:UniProtKB-KW P transcription, DNA-templated

REACTOME Pathway Links

REACTOME Pathway ID Description
5894006 Activation of gene expression by SREBF (SREBP)
5893843 PPARA activates gene expression
5893677 Transcriptional regulation of white adipocyte differentiation

Domain Information

InterPro Annotations

Accession Description
IPR020989 Histone-arginine methyltransferase CARM1, N-terminal
IPR025799 Protein arginine N-methyltransferase
IPR029063 S-adenosyl-L-methionine-dependent methyltransferase

UniProt Annotations

Entry Information

Gene Name
coactivator-associated arginine methyltransferase 1
Protein Entry
CARM1_MOUSE
UniProt ID
Q9WVG6
Species
Mouse

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q9WVG6-1; Sequence=Displayed; Name=2; IsoId=Q9WVG6-2; Sequence=VSP_012508; Note=No experimental confirmation available.;
Catalytic Activity S-adenosyl-L-methionine + arginine-[histone] = S-adenosyl-L-homocysteine + N(omega)-methyl-arginine-[histone]. {ECO:0000269|PubMed:11341840, ECO:0000269|PubMed:17882261, ECO:0000269|PubMed:19843527, ECO:0000269|PubMed:19897492, ECO:0000269|PubMed:21138967}.
Developmental Stage At E9, expression is prominent in the neural tube and somites. {ECO:0000269|PubMed:11713257}.
Disruption Phenotype Neonatal lethality. The lungs of neonates do not inflate and they do not breathe. The same neonate lethality is observed with mutants that produce CARM1 protein without enzyme activity. Embryos are distinctly smaller at 18.5 dpc. They show reduced lipid accumulation in brown adipose tissue and reduced amounts of brown adipose tissue. Thymocyte differentiation is blocked at an early stage. Mutants display complete loss of protein methylation of the CARM1 substrates PABPC1 and EP300/P300. {ECO:0000269|PubMed:12756295, ECO:0000269|PubMed:18188184, ECO:0000269|PubMed:19897492}.
Enzyme Regulation Methylation of H3R17 (H3R17me) by CARM1 is stimulated by preacetylation of H3 'Lys-18' (H3K18ac) H3 'Lys-23' (H3K23ac) by EP300 and blocked by citrullination of H3 'Arg-17' (H3R17ci) by PADI4. {ECO:0000269|PubMed:17882261}.
Function Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB- dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. {ECO:0000269|PubMed:10381882, ECO:0000269|PubMed:11341840, ECO:0000269|PubMed:11701890, ECO:0000269|PubMed:11713257, ECO:0000269|PubMed:11983685, ECO:0000269|PubMed:11997499, ECO:0000269|PubMed:12756295, ECO:0000269|PubMed:14966289, ECO:0000269|PubMed:15186775, ECO:0000269|PubMed:15616592, ECO:0000269|PubMed:16322096, ECO:0000269|PubMed:17218272, ECO:0000269|PubMed:17882261, ECO:0000269|PubMed:18188184, ECO:0000269|PubMed:19843527, ECO:0000269|PubMed:19897492, ECO:0000269|PubMed:21138967}.
Interaction P68433:Hist1h3i (xeno); NbExp=4; IntAct=EBI-4414343, EBI-1179609;
Ptm Auto-methylated on Arg-551. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing. {ECO:0000269|PubMed:10381882, ECO:0000269|PubMed:11701890, ECO:0000269|PubMed:11747826, ECO:0000269|PubMed:11751582, ECO:0000269|PubMed:11850402, ECO:0000269|PubMed:12237300, ECO:0000269|PubMed:12498683, ECO:0000269|PubMed:12756295, ECO:0000269|PubMed:15339660, ECO:0000269|PubMed:15616592, ECO:0000269|PubMed:21138967}.
Ptm Phosphorylation at Ser-217 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle (By similarity). Phosphorylation at Ser-217 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity. Phosphorylation at Ser-217 may promote cytosolic location. {ECO:0000250, ECO:0000269|PubMed:19843527}.
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family. {ECO:0000255|PROSITE-ProRule:PRU01015}.
Similarity Contains 1 SAM-dependent MTase PRMT-type domain. {ECO:0000255|PROSITE-ProRule:PRU01015}.
Subcellular Location Nucleus. Cytoplasm. Note=Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane (By similarity). {ECO:0000250}.
Subunit Homodimer. Interacts with NR1H4. Interacts with SNRPC (By similarity). Interacts with the C-terminus of NCOA2/GRIP1, NCO3/ACTR and NCOA1/SRC1. Part of a complex consisting of CARM1, EP300/P300 and NCOA2/GRIP1. Interacts with FLII, TP53, myogenic factor MEF2, EP300/P300, TRIM24, CREBBP and CTNNB1. Interacts with RELA. Identified in a complex containing CARM1, TRIM24 and NCOA2/GRIP1. Interacts with NCOA3/SRC3. {ECO:0000250, ECO:0000269|PubMed:10381882, ECO:0000269|PubMed:11701890, ECO:0000269|PubMed:11713257, ECO:0000269|PubMed:11983685, ECO:0000269|PubMed:11997499, ECO:0000269|PubMed:14966289, ECO:0000269|PubMed:15186775, ECO:0000269|PubMed:15616592, ECO:0000269|PubMed:16322096, ECO:0000269|PubMed:17882261, ECO:0000269|PubMed:19843527, ECO:0000269|PubMed:19897492}.
Tissue Specificity Ubiquitously expressed. Within the brain, present in proliferating cells from lateral ventricular zone and dentate gyrus (at protein level). {ECO:0000269|PubMed:10381882, ECO:0000269|PubMed:16508003}.

Identical and Related Proteins

Unique RefSeq proteins for LMP005596 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
50511310 RefSeq NP_067506 608 histone-arginine methyltransferase CARM1 isoform 1
189409145 RefSeq NP_694781 585 histone-arginine methyltransferase CARM1 isoform 2

Identical Sequences to LMP005596 proteins

Reference Database Accession Length Protein Name
GI:189409145 DBBJ BAE16336.1 585 coactivator-associated arginine methyltransferase 1 variant 4 [Rattus norvegicus]
GI:50511310 DBBJ BAE16333.1 608 coactivator-associated arginine methyltransferase 1 variant 1 [Rattus norvegicus]
GI:189409145 GenBank AAH36974.1 585 Carm1 protein [Mus musculus]
GI:50511310 GenBank AAD41265.2 608 protein arginine methyltransferase [Mus musculus]
GI:50511310 GenBank EDL78286.1 608 protein arginine N-methyltransferase 4 [Rattus norvegicus]
GI:50511310 GenBank ACQ18155.1 608 Sequence 69 from patent US 7510850
GI:50511310 RefSeq NP_001025212.1 608 histone-arginine methyltransferase CARM1 isoform a [Rattus norvegicus]
GI:189409145 RefSeq NP_001029260.1 585 histone-arginine methyltransferase CARM1 isoform d [Rattus norvegicus]
GI:50511310 SwissProt Q9WVG6.2 608 RecName: Full=Histone-arginine methyltransferase CARM1; AltName: Full=Coactivator-associated arginine methyltransferase 1; AltName: Full=Protein arginine N-methyltransferase 4 [Mus musculus]

Related Sequences to LMP005596 proteins

Reference Database Accession Length Protein Name
GI:189409145 DBBJ BAE16333.1 608 coactivator-associated arginine methyltransferase 1 variant 1 [Rattus norvegicus]
GI:189409145 GenBank AAD41265.2 608 protein arginine methyltransferase [Mus musculus]
GI:50511310 GenBank AAV01036.1 608 Sequence 2 from patent US 6743614
GI:50511310 GenBank ABH83682.1 608 Sequence 8 from patent US 7049088
GI:189409145 GenBank EDL78286.1 608 protein arginine N-methyltransferase 4 [Rattus norvegicus]
GI:189409145 GenBank ACQ18155.1 608 Sequence 69 from patent US 7510850
GI:50511310 GenBank AFG96339.1 608 Sequence 2 from patent US 8148124
GI:50511310 GenBank AFG96340.1 608 Sequence 3 from patent US 8148124
GI:50511310 GenBank AFQ82086.1 608 Sequence 2 from patent US 8247168
GI:50511310 GenBank AID68328.1 615 coactivator-associated arginine methyltransferase 1 variant 5 [Rattus norvegicus]
GI:189409145 RefSeq NP_067506.2 608 histone-arginine methyltransferase CARM1 isoform 1 [Mus musculus]
GI:189409145 RefSeq NP_001025212.1 608 histone-arginine methyltransferase CARM1 isoform a [Rattus norvegicus]