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Catalytic Activity
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Dolichyl diphosphooligosaccharide + [protein]- L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.
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Function
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Essential subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
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Interaction
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P52870:SBH1; NbExp=2; IntAct=EBI-12673, EBI-16410; P32915:SEC61; NbExp=2; IntAct=EBI-12673, EBI-16400;
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Pathway
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Protein modification; protein glycosylation.
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Sequence Caution
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Sequence=CAA64024.1; Type=Erroneous initiation; Evidence= ; Sequence=CAA99300.1; Type=Erroneous initiation; Evidence= ;
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Sequence Caution
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Sequence=CAA64024.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAA99300.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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Similarity
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Belongs to the DAD/OST2 family
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Similarity
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Belongs to the DAD/OST2 family. {ECO:0000305}.
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Subcellular Location
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Endoplasmic reticulum membrane; Multi-pass membrane protein.
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Subunit
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Component of the oligosaccharyltransferase (OST) complex, which appears to exist in two assemblies comprising OST1, OST2, OST4, OST5, STT3, SWP1, WPB1, and either OST3 or OST6. WBP1, SWP1 and OST2 probably form a subcomplex. May interact directly with OST1, OST3, OST4, OST5, OST6, WBP1 and SWP1. Interacts with SEC61 and SSS1. {ECO:0000269|PubMed:15831493, ECO:0000269|PubMed:15886282, ECO:0000269|PubMed:8175708, ECO:0000269|PubMed:9405463}.
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