LIPID MAPS

LMPD ID

LMP007155

Gene ID

856366

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

Opi1p

Gene Symbol

OPI1

Synonyms

-

Chromosome

VIII

Opi1p
Refseq ID	NP_011843
Protein GI	6321767
UniProt ID	P21957
mRNA ID	NM_001179100
Length	404
MSENQRLGLSEEEVEAAEVLGVLKQSCRQKSQPSEDVSQADKMPASESSTTPLNILDRVSNKIISNVVTFYDEINTNKRPLKSIGRLLDDDDDEHDDYDYNDDEFFTNKRQKLSRAIAKGKDNLKEYKLNMSIESKKRLVTCLHLLKLANKQLSDKISCLQDLVEKEQVHPLHKQDGNARTTTGAGEDETSSDEDDDDEEFFDASEQVNASEQSIVVKMEVVGTVKKVYSLISKFTANSLPEPARSQVRESLLNLPTNWFDSVHSTSLPHHASFHYANCEEQKVEQQQQQQQQQQQQQLLQQQLLQQQQQKRNKDGDDSASPSSSVTANGKVLILAKESLEMVRNVMGVVDSTLGKAEEWVKQKQEVKEMIRERFLQQQQQYRQQQQKDGNYVKPSQDNVDSKD

Entrez Gene ID

856366

Gene Name

Opi1p

Gene Symbol

OPI1

Species

Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID	Source	Type	Description
GO:0005783	IDA:SGD	C	endoplasmic reticulum
GO:0005635	IDA:SGD	C	nuclear envelope
GO:0031965	IDA:SGD	C	nuclear membrane
GO:0005654	IDA:SGD	C	nucleoplasm
GO:0005634	IDA:SGD	C	nucleus
GO:0003677	IEA:UniProtKB-KW	F	DNA binding
GO:0003714	IPI:SGD	F	transcription corepressor activity
GO:0030968	IMP:SGD	P	endoplasmic reticulum unfolded protein response
GO:0000122	IMP:SGD	P	negative regulation of transcription from RNA polymerase II promoter
GO:0008654	IMP:SGD	P	phospholipid biosynthetic process
GO:0045944	IMP:SGD	P	positive regulation of transcription from RNA polymerase II promoter
GO:0006351	IEA:UniProtKB-KW	P	transcription, DNA-templated

InterPro Annotations

Accession	Description
IPR013927	TF_Opi1

Entry Information

Gene Name

Opi1p

Protein Entry

OPI1_YEAST

UniProt ID

P21957

Species

Yeast (S288c)

Comments

Comment Type	Description
Domain	The FFAT motif is required for interaction with SCS2 and proper localization of the protein
Domain	The FFAT motif is required for interaction with SCS2 and proper localization of the protein. {ECO:0000269\|PubMed:12727870}.
Function	Negative regulator of the transcriptional complex INO2- INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis
Function	Negative regulator of the transcriptional complex INO2- INO4 in response to phospholipid precursor availability. When precursors become limiting, OPI1 is retained at the endoplasmic reticulum (ER) and INO2-INO4 activates INO1 and other genes required for phospholipid biosynthesis, whereas abundant precursor availability results in targeting of OPI1 to the nucleus to repress transcription of these genes. Binds directly to phosphatidic acid, which is required for ER targeting and may act as sensing mechanism for precursor availability, as phosphatidic acid becomes rapidly depleted upon phospholipid biosynthesis. {ECO:0000269\|PubMed:15192221}.
Interaction	P22579:SIN3; NbExp=5; IntAct=EBI-12555, EBI-17160;
Miscellaneous	Present with 1281 molecules/cell in log phase SD medium
Miscellaneous	Present with 1281 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Subcellular Location	Endoplasmic reticulum. Nucleus. Note=Maintained at the endoplasmic reticulum by SCS2. In response to elevated inositol levels.
Subunit	Interacts with SCS2. {ECO:0000269\|PubMed:12727870, ECO:0000269\|PubMed:15455074}.