Gene/Proteome Database (LMPD)

LMPD ID
LMP007195
Gene ID
854762
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
Dfg10p
Gene Symbol
DFG10
Synonyms
-
Alternate Names
Dfg10p
Chromosome
IX
EC Number
1.3.1.94

Proteins

Dfg10p
Refseq ID NP_012215
Protein GI 6322140
UniProt ID P40526
mRNA ID NM_001179399
Length 253
RefSeq Status PROVISIONAL
MYFDEEQLLKYTIYAYRLSFFVGICSLFIAKSCLPEFLQYGKTYRPKENSKYSSILERIKKFTVPKAYFSHFYYLATFLSLVTLYFYPKFPIVWIIFGHSLRRLYETLYVLHYTSNSRMNWSHYLVGIWFYSVLLLILNISLYKNSIPNTLNMNAFIIFCIASWDQYKNHVILANLVKYSLPTGRLFRLVCCPHYLDEIIIYSTLLPYEQEFYLTLVWVITSLTISALETKNYYRHKFKDNHVAPYAIIPFII

Gene Information

Entrez Gene ID
854762
Gene Name
Dfg10p
Gene Symbol
DFG10
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0003865 ISS:SGD F 3-oxo-5-alpha-steroid 4-dehydrogenase activity
GO:0019408 IMP:SGD P dolichol biosynthetic process
GO:0019348 IMP:UniProtKB P dolichol metabolic process
GO:0006488 IMP:UniProtKB P dolichol-linked oligosaccharide biosynthetic process
GO:0016095 IMP:UniProtKB P polyprenol catabolic process
GO:0006486 IEA:UniProtKB-UniPathway P protein glycosylation
GO:0007124 IMP:SGD P pseudohyphal growth

Domain Information

InterPro Annotations

Accession Description
IPR001104 3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal

UniProt Annotations

Entry Information

Gene Name
Dfg10p
Protein Entry
DFG10_YEAST
UniProt ID
P40526
Species
Yeast (S288c)

Comments

Comment Type Description
Catalytic Activity Ditrans,polycis-dolichol + NADP(+) = ditrans,polycis-polyprenol + NADPH. {ECO:0000269|PubMed:20637498}.
Disruption Phenotype Suppression of filamentatous growth, defects in cell polarity, and cellular elongation, due to defects in N- glycosylation. {ECO:0000269|PubMed:20637498, ECO:0000269|PubMed:9055077}.
Function Plays a key role in early steps of protein N-linked glycosylation by being required for the conversion of polyprenol into dolichol. Dolichols are required for the synthesis of dolichol-linked monosaccharides and the oligosaccharide precursor used for N-glycosylation. Acts as a polyprenol reductase that promotes the reduction of the alpha-isoprene unit of polyprenols into dolichols in a NADP-dependent mechanism. {ECO:0000269|PubMed:20637498}.
Pathway Protein modification; protein glycosylation.
Similarity Belongs to the steroid 5-alpha reductase family. Polyprenol reductase subfamily. {ECO:0000305}.
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007195 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
6322140 RefSeq NP_012215 253 Dfg10p

Identical Sequences to LMP007195 proteins

Reference Database Accession Length Protein Name
GI:6322140 EMBL CAA86173.1 253 unnamed protein product [Saccharomyces cerevisiae]
GI:6322140 GenBank EDN61445.1 253 conserved protein [Saccharomyces cerevisiae YJM789]
GI:6322140 SwissProt P40526.1 253 RecName: Full=Polyprenol reductase; AltName: Full=Protein DFG10 [Saccharomyces cerevisiae S288c]
GI:6322140 Third Party Genbank DAA08498.1 253 TPA: Dfg10p [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007195 proteins

Reference Database Accession Length Protein Name
GI:6322140 EMBL CAY80460.1 253 Dfg10p [Saccharomyces cerevisiae EC1118]
GI:6322140 GenBank EDZ71497.1 253 YIL049Wp-like protein [Saccharomyces cerevisiae AWRI1631]
GI:6322140 GenBank EEU05062.1 253 Dfg10p [Saccharomyces cerevisiae JAY291]
GI:6322140 GenBank EGA78452.1 253 Dfg10p [Saccharomyces cerevisiae Vin13]
GI:6322140 GenBank EGA86438.1 253 Dfg10p [Saccharomyces cerevisiae VL3]
GI:6322140 gnl McCuskerlabDuke 253 Dfg10p [Saccharomyces cerevisiae YJM993]