Gene/Proteome Database (LMPD)

LMPD ID
LMP007346
Gene ID
853148
Species
Saccharomyces cerevisiae S288c (Yeast (S288c))
Gene Name
Pho81p
Gene Symbol
PHO81
Synonyms
VAC6
Alternate Names
Pho81p
Chromosome
VII

Proteins

Pho81p
Refseq ID NP_011749
Protein GI 398366247
UniProt ID P17442
mRNA ID NM_001181362
Length 1178
RefSeq Status PROVISIONAL
MKFGKYLEARQLELAEYNSHFIDYKALKKLIKQLAIPTLKASSDLDLHLTLDDIDEKIIHQRLQENKAAFFFKLERELEKVNGYYLARESDLRIKFNILHSKYKDYKINGKLNSNQATSFKNLYAAFKKFQKDLRNLEQYVELNKTGFSKALKKWDKRSQSHDKDFYLATVVSIQPIFTRDGPLKLNDETLHILLELNDIDNNNRRADIQSSTFTNDDDDDNNTSNNNKHNNNNNNNNNNNNNNNNNNILHNNYELTTSKISENQLEHLFQASSSSLDMEMEIENWYKEILNIATVKDVQRKHALLRNFRETKIFTYLLQNSSESFHKNVFSLLKECLTTLFLLLVASPLDDNSLHIFYKSNQDHIDLSYCDEDDQVFSRKNVFHEAASCPEKSRLFILDEALTTSKLSKETVQKLLNAQDIHSRVPLHYAAELGKLEFVHSLLITNLLEDVDPIDSDSKTPLVLAITNNHIDVVRDLLTIGGANASPIEKPILDYSKNVISSTKVQFDPLNVACKFNNHDAAKLLLEIRSKQNADNAKNKSSQHLCQPLFKKNSTGLCTLHIVAKIGGDPQLIQLLIRYGADPNEIDGFNKWTPIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALLQRPLNLPSAPLNEINSQSSTQRLNTIDLTPNDDKFDLDIQDSIPDFALPPPIIPLRKYGHNFLEKKIFIKLKLRPGLESIKLTQDNGIIMSSSPGRITLSSNLPEIIPRNVILPVRSGEINNFCKDISETNDEEDDDEISEDHDDGEIIFQVDSIDDFSMDFEIFPSFGTRIIAKTTAMPFLFKKVAINSIATMNLPLFDTRLNNIGSLTLDYQIIFPYPGNPLKIINYEPYWKSTGSDLMTSSKDGNFVTSSSLNGSFISVLVCALNDETIVAAPKPYVEFKGTKILLNDLTKEQLEKVVDYDFGKIDGSFDEVTLKQYLSSRVVPLRSLLEVIPGSAQLVIRVYFPTDKEIDTIPIKISPFININQFIDKLLLIIFEHERFLRHSGSGSMRQIVFSSCNWEACSILNWKQPNFPVLLQMKNLLRDSTTGKFVGDTPNCLKELAVNPQKMSYLNTELINIHTMVQFAMNNNLLGVTLPYEVLKICPSLARIIKQNGLLLIASVGENDQIPADGGYSGIYYACELLFENNIDM

Gene Information

Entrez Gene ID
853148
Gene Name
Pho81p
Gene Symbol
PHO81
Species
Saccharomyces cerevisiae S288c

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005737 IEA:UniProtKB-KW C cytoplasm
GO:0005634 IDA:SGD C nucleus
GO:0004861 IMP:SGD F cyclin-dependent protein serine/threonine kinase inhibitor activity
GO:0008081 IEA:InterPro F phosphoric diester hydrolase activity
GO:0006629 IEA:InterPro P lipid metabolic process
GO:0071901 IMP:GOC P negative regulation of protein serine/threonine kinase activity
GO:0006796 IMP:SGD P phosphate-containing compound metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04111 Cell cycle - yeast
sce04111 Cell cycle - yeast

Domain Information

InterPro Annotations

Accession Description
IPR002110 Ankyrin repeat
IPR020683 Ankyrin repeat-containing domain
IPR017946 PLC-like phosphodiesterase, TIM beta/alpha-barrel domain
IPR004331 SPX, N-terminal

UniProt Annotations

Entry Information

Gene Name
Pho81p
Protein Entry
PHO81_YEAST
UniProt ID
P17442
Species
Yeast (S288c)

Comments

Comment Type Description
Function Inhibits the kinase activity of the cyclin-CDKs PHO80- PHO85 and PCL7-PHO85 under low-phosphate conditions. {ECO:0000269|PubMed:11069666, ECO:0000269|PubMed:7939631, ECO:0000269|PubMed:7957107}.
Interaction P17157:PHO85; NbExp=5; IntAct=EBI-13314, EBI-13327;
Miscellaneous Present with 2930 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.
Ptm Phosphorylated by the cyclin-CDK PHO80-PHO85. Phosphorylation mediates the formation of a stable interaction with the cyclin-CDK and is required for function as an active inhibitor of the complex under phosphate starvation conditions. {ECO:0000269|PubMed:15057567, ECO:0000269|PubMed:15127225, ECO:0000269|PubMed:18407956}.
Similarity Contains 1 GP-PDE domain. {ECO:0000305}.
Similarity Contains 1 SPX domain. {ECO:0000255|PROSITE- ProRule:PRU00714}.
Similarity Contains 6 ANK repeats. {ECO:0000255|PROSITE- ProRule:PRU00023}.
Subcellular Location Cytoplasm. Nucleus. Note=Localizes predominantly to the nucleus in both high- and low-phosphate conditions.
Subunit Associates specifically with the PHO80-PHO85 and PCL7- PHO85 cyclin-CDK complexes, and much of this interaction is mediated through the PHO80 and PCL7 cyclin subunits. Interacts with the transcription factor PHO4. {ECO:0000269|PubMed:11069666, ECO:0000269|PubMed:7939631, ECO:0000269|PubMed:7957107}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007346 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
398366247 RefSeq NP_011749 1178 Pho81p

Identical Sequences to LMP007346 proteins

Reference Database Accession Length Protein Name
GI:398366247 EMBL CAA61183.1 1178 ORF 1178 [Saccharomyces cerevisiae]
GI:398366247 EMBL CAA97261.1 1178 PHO81 [Saccharomyces cerevisiae]
GI:398366247 GenBank ABZ48059.1 1178 Sequence 21997 from patent US 7314974
GI:398366247 GenBank EIW10580.1 1178 Pho81p [Saccharomyces cerevisiae CEN.PK113-7D]
GI:398366247 SwissProt P17442.2 1178 RecName: Full=Phosphate system positive regulatory protein PHO81; AltName: Full=CDK inhibitor PHO81 [Saccharomyces cerevisiae S288c]
GI:398366247 Third Party Genbank DAA08324.1 1178 TPA: Pho81p [Saccharomyces cerevisiae S288c]

Related Sequences to LMP007346 proteins

Reference Database Accession Length Protein Name
GI:398366247 DBBJ BAA02508.1 1179 PHO81 [Saccharomyces cerevisiae]
GI:398366247 DBBJ GAA23602.1 1179 K7_Pho81p [Saccharomyces cerevisiae Kyokai no. 7]
GI:398366247 EMBL CAA36726.1 1177 unnamed protein product [Saccharomyces cerevisiae]
GI:398366247 EMBL CAY79991.1 1177 Pho81p [Saccharomyces cerevisiae EC1118]
GI:398366247 GenBank EEU07614.1 1177 Pho81p [Saccharomyces cerevisiae JAY291]
GI:398366247 gnl McCuskerlabDuke 1177 Pho81p [Saccharomyces cerevisiae YJM993]