LIPID MAPS

LMPD ID

LMP007424

Gene ID

Species

Saccharomyces cerevisiae S288c (Yeast (S288c))

Gene Name

diacylglycerol O-acyltransferase

Gene Symbol

DGA1

Synonyms

-

Chromosome

XV

EC Number

2.3.1.20

diacylglycerol O-acyltransferase
Refseq ID	NP_014888
Protein GI	6324819
UniProt ID	Q08650
mRNA ID	NM_001183664
Length	418
MSGTFNDIRRRKKEEGSPTAGITERHENKSLSSIDKREQTLKPQLESCCPLATPFERRLQTLAVAWHTSSFVLFSIFTLFAISTPALWVLAIPYMIYFFFDRSPATGEVVNRYSLRFRSLPIWKWYCDYFPISLIKTVNLKPTFTLSKNKRVNEKNYKIRLWPTKYSINLKSNSTIDYRNQECTGPTYLFGYHPHGIGALGAFGAFATEGCNYSKIFPGIPISLMTLVTQFHIPLYRDYLLALGISSVSRKNALRTLSKNQSICIVVGGARESLLSSTNGTQLILNKRKGFIKLAIQTGNINLVPVFAFGEVDCYNVLSTKKDSVLGKMQLWFKENFGFTIPIFYARGLFNYDFGLLPFRAPINVVVGRPIYVEKKITNPPDDVVNHFHDLYIAELKRLYYENREKYGVPDAELKIVG

Entrez Gene ID

Gene Name

diacylglycerol O-acyltransferase

Gene Symbol

DGA1

Species

Saccharomyces cerevisiae S288c

GO ID	Source	Type	Description
GO:0005783	IEA:UniProtKB-KW	C	endoplasmic reticulum
GO:0016021	IDA:SGD	C	integral component of membrane
GO:0005811	IDA:SGD	C	lipid particle
GO:0003846	IEA:UniProtKB-EC	F	2-acylglycerol O-acyltransferase activity
GO:0004144	IDA:SGD	F	diacylglycerol O-acyltransferase activity
GO:0006672	IDA:SGD	P	ceramide metabolic process
GO:0006071	IEA:UniProtKB-KW	P	glycerol metabolic process
GO:0019915	IMP:SGD	P	lipid storage
GO:0019432	IDA:SGD	P	triglyceride biosynthetic process

BIOCYC Pathway ID	Description
PWY-7420	monoacylglycerol metabolism
PWY-7420	monoacylglycerol metabolism (yeast)
TRIGLSYN-PWY	triacylglycerol biosynthesis
TRIGLSYN-PWY	triacylglycerol biosynthesis

REACTOME Pathway ID	Description
5618590	Acyl chain remodeling of DAG and TAG
5618090	Fatty acid, triacylglycerol, and ketone body metabolism
5618125	Glycerophospholipid biosynthesis
5618023	Metabolism
5618091	Metabolism of lipids and lipoproteins
5618126	Phospholipid metabolism
5618115	Triglyceride Biosynthesis

Accession	Description
IPR007130	Diacylglycerol acyltransferase

Gene Name

diacylglycerol O-acyltransferase

Protein Entry

DGAT2_YEAST

UniProt ID

Species

Yeast (S288c)

Comment Type	Description
Catalytic Activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
Catalytic Activity	Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol. {ECO:0000269\|PubMed:20554061}.
Catalytic Activity	Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol
Catalytic Activity	Acyl-CoA + 2-acylglycerol = CoA + diacylglycerol. {ECO:0000269\|PubMed:20554061}.
Function	Catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. Required for storage lipid synthesis. May be involved in lipid particle synthesis from the endoplasmic reticulum and ergosterol biosynthesis. Also has monoacylglycerol acyltransferase (MGAT) activity, catalyzing the acyl-CoA-dependent esterification of monoacylglycerol to diacylglycerol. {ECO:0000269\|PubMed:11741946, ECO:0000269\|PubMed:11751830, ECO:0000269\|PubMed:11751875, ECO:0000269\|PubMed:14640980, ECO:0000269\|PubMed:15155725, ECO:0000269\|PubMed:21321129}.
Interaction	P06169:PDC1; NbExp=1; IntAct=EBI-33586, EBI-5687;
Miscellaneous	Present with 907 molecules/cell in log phase SD medium
Miscellaneous	Present with 907 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Pathway	Glycerolipid metabolism; triacylglycerol biosynthesis.
Similarity	Belongs to the diacylglycerol acyltransferase family
Similarity	Belongs to the diacylglycerol acyltransferase family. {ECO:0000305}.
Subcellular Location	Endoplasmic reticulum membrane; Multi-pass membrane protein. Lipid droplet.