Gene/Proteome Database (LMPD)

LMPD ID
LMP007582
Gene ID
948269
Species
Escherichia coli K-12 (E. coli)
Gene Name
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
Gene Symbol
ispD
Synonyms
ECK2742; JW2717; ygbP
Summary
IspD is also called 4-diphosphocytidyl-2-C-methylerythritol synthase. [More information is available at EcoGene: EG13110]. 4-diphosphocytidyl-2C-methyl-D-erythritol synthase (IspD) catalyzes the third step in the methylerythritol phosphate pathway. [More information is available at EcoCyc: G7423].
Orthologs
View orthologs and multiple alignments for ispD

Proteins

4-diphosphocytidyl-2C-methyl-D-erythritol synthase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_417227
Protein GI 16130654
UniProt ID Q46893
Length 236
RefSeq Status REVIEWED
MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHALLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWVLVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGLWHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPEDLALAEFYLTRTIHQENT

Gene Information

Entrez Gene ID
948269
Gene Name
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
Gene Symbol
ispD
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0050518 IDA:EcoCyc F 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity
GO:0042802 IDA:EcoCyc F identical protein binding
GO:0000287 IDA:EcoCyc F magnesium ion binding
GO:0019288 IEA:UniProtKB-UniPathway P isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0016114 IEA:UniProtKB-HAMAP P terpenoid biosynthetic process
GO:0006744 IDA:EcoCyc P ubiquinone biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco_M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco01100 Metabolic pathways
eco00900 Terpenoid backbone biosynthesis
ko00900 Terpenoid backbone biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
NONMEVIPP-PWY methylerythritol phosphate pathway
NONMEVIPP-PWY methylerythritol phosphate pathway
NONMEVIPP-PWY methylerythritol phosphate pathway
PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PWY-5121 superpathway of geranylgeranyldiphosphate biosynthesis II (via MEP)
PWY-7392 taxadiene biosynthesis (engineered)

Domain Information

InterPro Annotations

Accession Description
IPR001228 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
IPR018294 4-diphosphocytidyl-2C-methyl-D-erythritol synthase, conserved site
IPR029044 Nucleotide-diphospho-sugar transferases

UniProt Annotations

Entry Information

Gene Name
4-diphosphocytidyl-2C-methyl-D-erythritol synthase
Protein Entry
ISPD_ECOLI
UniProt ID
Q46893
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties pH dependence: Optimum pH is 8.3.;
Catalytic Activity CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Name=Co(2+); Xref=ChEBI:CHEBI:48828;
Function Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP).
Miscellaneous There are no coordination bonds that occur between IspD and magnesium in any of the complexes examined to date.
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 2/6.
Similarity Belongs to the IspD family. {ECO:0000305}.
Subunit Homodimer. {ECO:0000269|PubMed:11427897}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007582 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16130654 RefSeq NP_417227 236 4-diphosphocytidyl-2C-methyl-D-erythritol synthase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007582 proteins

Reference Database Accession Length Protein Name
GI:16130654 GenBank KHJ01534.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli]
GI:16130654 GenBank KHJ04847.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli]
GI:16130654 GenBank KHJ12319.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli]
GI:16130654 GenBank KHJ16625.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli]
GI:16130654 GenBank KHJ29325.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli]
GI:16130654 gnl IGS 236 4-diphosphocytidyl-2C-methyl-D-erythritol synthase [Escherichia coli ER2796]

Related Sequences to LMP007582 proteins

Reference Database Accession Length Protein Name
GI:16130654 GenBank EGI35067.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli TA271]
GI:16130654 GenBank EGI44878.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli H591]
GI:16130654 GenBank EGW81219.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli STEC_94C]
GI:16130654 GenBank ELG68354.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli KTE136]
GI:16130654 GenBank EMX14114.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli P0302293.2]
GI:16130654 GenBank EOU31638.1 236 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase [Escherichia coli KTE13]