Gene/Proteome Database (LMPD)

LMPD ID
LMP007586
Gene ID
948789
Species
Escherichia coli K-12 (E. coli)
Gene Name
UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase
Gene Symbol
wecA
Synonyms
ECK3776; JW3758; rfe
Summary
None
Orthologs
View orthologs and multiple alignments for wecA

Proteins

UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_418231
Protein GI 16131640
UniProt ID P0AC78
Length 367
RefSeq Status REVIEWED
MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQGLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRATIQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDGLLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDAGSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPDRQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLYGYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK

Gene Information

Entrez Gene ID
948789
Gene Name
UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase
Gene Symbol
wecA
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0009276 IDA:UniProtKB C Gram-negative-bacterium-type cell wall
GO:0016021 IDA:EcoliWiki C integral component of membrane
GO:0005887 IMP:UniProtKB C integral component of plasma membrane
GO:0016020 IDA:EcoCyc C membrane
GO:0036380 IEA:UniProtKB-EC F UDP-N-acetylglucosamine-undecaprenyl-phosphate N-acetylglucosaminephosphotransferase activity
GO:0008144 IDA:EcoliWiki F drug binding
GO:0042802 IDA:EcoCyc F identical protein binding
GO:0000287 IDA:UniProtKB F magnesium ion binding
GO:0030145 IDA:UniProtKB F manganese ion binding
GO:0008963 IDA:EcoliWiki F phospho-N-acetylmuramoyl-pentapeptide-transferase activity
GO:0016780 IDA:UniProtKB F phosphotransferase activity, for other substituted phosphate groups
GO:0016757 IEA:UniProtKB-KW F transferase activity, transferring glycosyl groups
GO:0009243 IEA:UniProtKB-UniPathway P O antigen biosynthetic process
GO:0044038 IDA:UniProtKB P cell wall macromolecule biosynthetic process
GO:0071555 IDA:UniProtKB P cell wall organization
GO:0009246 IMP:EcoCyc P enterobacterial common antigen biosynthetic process
GO:0009103 IDA:UniProtKB P lipopolysaccharide biosynthetic process

BIOCYC Pathway Links

BIOCYC Pathway ID Description
ECASYN-PWY enterobacterial common antigen biosynthesis
ECASYN-PWY enterobacterial common antigen biosynthesis
ECASYN-PWY enterobacterial common antigen biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR012750 ECA_WecA-rel
IPR000715 Glycosyl transferase, family 4

UniProt Annotations

Entry Information

Gene Name
UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase
Protein Entry
WECA_ECOLI
UniProt ID
P0AC78
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=5.6 uM for UDP-GlcNAc {ECO:0000269|PubMed:9134438};
Catalytic Activity UDP-N-acetyl-alpha-D-glucosamine + ditrans,octacis-undecaprenyl phosphate = UMP + N-acetyl-alpha-D- glucosaminyl-diphospho-ditrans,octacis-undecaprenol.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:17237164};
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:17237164};
Enzyme Regulation Inhibited by tunicamycin.
Function Catalyzes the transfer of the GlcNAc-1-phosphate moiety from UDP-GlcNAc onto the carrier lipid undecaprenyl phosphate (C55-P), yielding GlcNAc-pyrophosphoryl-undecaprenyl (GlcNAc-PP- C55). It is the first lipid-linked intermediate involved in enterobacterial common antigen (ECA) synthesis and an acceptor for the addition of subsequent sugars to complete the biosynthesis of O-antigen. The apparent affinity of WecA for the polyisoprenyl phosphate substrates increases with the polyisoprenyl chain length. WecA is unable to utilize dolichyl phosphate (Dol-P). {ECO:0000269|PubMed:11700352, ECO:0000269|PubMed:1730666}.
Pathway Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.
Sequence Caution Sequence=AAA24526.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAB20842.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};
Similarity Belongs to the glycosyltransferase 4 family. WecA subfamily. {ECO:0000305}.
Subcellular Location Cell inner membrane; Multi-pass membrane protein.

Identical and Related Proteins

Unique RefSeq proteins for LMP007586 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16131640 RefSeq NP_418231 367 UDP-GlcNAc:undecaprenylphosphate GlcNAc-1-phosphate transferase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007586 proteins

Reference Database Accession Length Protein Name
GI:16131640 GenBank KHJ03930.1 367 UDP-phosphate N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 GenBank KHJ09496.1 367 UDP-phosphate N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 GenBank KHJ13147.1 367 UDP-phosphate N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 GenBank KHJ17226.1 367 UDP-phosphate N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 GenBank KHJ23710.1 367 UDP-phosphate N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 GenBank KHJ29419.1 367 UDP-phosphate N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]

Related Sequences to LMP007586 proteins

Reference Database Accession Length Protein Name
GI:16131640 GenBank EHW44967.1 367 undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase [Escherichia coli DEC9A]
GI:16131640 GenBank EHW50792.1 367 undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase [Escherichia coli DEC9E]
GI:16131640 GenBank EKI48623.1 367 undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphatetransferase [Escherichia coli N1]
GI:16131640 RefSeq WP_001050958.1 367 UDP-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 RefSeq WP_001050962.1 367 UDP-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase [Escherichia coli]
GI:16131640 RefSeq WP_001050963.1 367 UDP-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase [Escherichia albertii]