Gene/Proteome Database (LMPD)
LMPD ID
LMP007603
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Gene Symbol
Synonyms
ECK2335; JW2338; yfcX
Summary
Also known as beta-oxidation complex II. [More information is available at EcoGene: EG14127]. During anaerobic beta-oxidation of fatty acids FadI, FadJ, and FadK serve functions parallel to those of FadA, FadB, and FadD in the aerobic pathway . [More information is available at EcoCyc: G7212].
Orthologs
Proteins
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli str. K-12 substr. MG1655] | |
---|---|
Refseq ID | NP_416843 |
Protein GI | 16130274 |
UniProt ID | P77399 |
Length | 714 |
RefSeq Status | REVIEWED |
MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIKQLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPIQVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIGVSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKERPSSRPLPVRERILAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGELAMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGIPVRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLIIEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSPVEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIRMLTQGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVVSSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGTQGQGRISAPQVAERCVMLMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLATQYGSRFTPCERLVEMGARGESFWKTTATDLQ |
Gene Information
Entrez Gene ID
Gene Name
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Gene Symbol
Species
Escherichia coli K-12
Gene Ontology (GO Annotations)
GO ID | Source | Type | Description |
---|---|---|---|
GO:0005737 | IEA:UniProtKB-HAMAP | C | cytoplasm |
GO:0003857 | IEA:UniProtKB-HAMAP | F | 3-hydroxyacyl-CoA dehydrogenase activity |
GO:0008692 | IEA:UniProtKB-HAMAP | F | 3-hydroxybutyryl-CoA epimerase activity |
GO:0051287 | IEA:InterPro | F | NAD binding |
GO:0004300 | IEA:UniProtKB-HAMAP | F | enoyl-CoA hydratase activity |
GO:0006635 | IEA:UniProtKB-UniPathway | P | fatty acid beta-oxidation |
KEGG Pathway Links
KEGG Pathway ID | Description |
---|---|
eco01110 | Biosynthesis of secondary metabolites |
eco01040 | Biosynthesis of unsaturated fatty acids |
ko01040 | Biosynthesis of unsaturated fatty acids |
eco00650 | Butanoate metabolism |
ko00650 | Butanoate metabolism |
eco00930 | Caprolactam degradation |
ko00930 | Caprolactam degradation |
eco01200 | Carbon metabolism |
ko01200 | Carbon metabolism |
eco00071 | Fatty acid degradation |
ko00071 | Fatty acid degradation |
eco01212 | Fatty acid metabolism |
ko01212 | Fatty acid metabolism |
eco00281 | Geraniol degradation |
ko00281 | Geraniol degradation |
eco00903 | Limonene and pinene degradation |
ko00903 | Limonene and pinene degradation |
eco00310 | Lysine degradation |
ko00310 | Lysine degradation |
eco01100 | Metabolic pathways |
eco01120 | Microbial metabolism in diverse environments |
eco00640 | Propanoate metabolism |
ko00640 | Propanoate metabolism |
eco00380 | Tryptophan metabolism |
ko00380 | Tryptophan metabolism |
eco00280 | Valine, leucine and isoleucine degradation |
ko00280 | Valine, leucine and isoleucine degradation |
eco00410 | beta-Alanine metabolism |
ko00410 | beta-Alanine metabolism |
M00087 | beta-Oxidation |
eco_M00087 | beta-Oxidation |
BIOCYC Pathway Links
Domain Information
InterPro Annotations
Accession | Description |
---|---|
IPR006108 | 3-hydroxyacyl-CoA dehydrogenase, C-terminal |
IPR006176 | 3-hydroxyacyl-CoA dehydrogenase, NAD binding |
IPR006180 | 3-hydroxyacyl-CoA dehydrogenase, conserved site |
IPR008927 | 6-phosphogluconate dehydrogenase, C-terminal-like |
IPR029045 | ClpP/crotonase-like domain |
IPR001753 | Crotonase_core_superfam |
IPR013328 | Dehydrogenase, multihelical |
IPR012802 | FadJ |
IPR016040 | NAD(P)-binding domain |
UniProt Annotations
Entry Information
Gene Name
enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase
Protein Entry
FADJ_ECOLI
UniProt ID
Species
E. coli
Comments
Comment Type | Description |
---|---|
Catalytic Activity | (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl- CoA + H(2)O. |
Catalytic Activity | (S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH. |
Catalytic Activity | (S)-3-hydroxybutanoyl-CoA = (R)-3- hydroxybutanoyl-CoA. |
Function | Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities. Strongly involved in the anaerobic degradation of long and medium-chain fatty acids in the presence of nitrate and weakly involved in the aerobic degradation of long-chain fatty acids. {ECO:0000269|PubMed:12270828, ECO:0000269|PubMed:12535077}. |
Induction | Unlike the aerobic pathway, the anaerobic pathway is not strongly repressed by FadR regulatory protein. |
Pathway | Lipid metabolism; fatty acid beta-oxidation. |
Similarity | In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. {ECO:0000305}. |
Similarity | In the central section; belongs to the 3-hydroxyacyl- CoA dehydrogenase family. {ECO:0000305}. |
Subcellular Location | Cytoplasm {ECO:0000250}. |
Subunit | Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI). {ECO:0000250}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP007603 (as displayed in Record Overview)
Protein GI | Database | Accession | Length | Protein Name |
---|---|---|---|---|
16130274 | RefSeq | NP_416843 | 714 | enoyl-CoA hydratase/epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli str. K-12 substr. MG1655] |
Identical Sequences to LMP007603 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:16130274 | GenBank | KHD59173.1 | 714 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
GI:16130274 | GenBank | KHH68448.1 | 714 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
GI:16130274 | GenBank | KHH89814.1 | 714 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
GI:16130274 | GenBank | KHI21516.1 | 714 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
GI:16130274 | GenBank | KHI60669.1 | 714 | multifunctional fatty acid oxidation complex subunit alpha [Escherichia coli] |
GI:16130274 | gnl | IGS | 714 | fused enoyl-CoA hydratase and epimerase and isomerase/3-hydroxyacyl-CoA dehydrogenase [Escherichia coli ER2796] |
Related Sequences to LMP007603 proteins
Reference | Database | Accession | Length | Protein Name |
---|---|---|---|---|
GI:16130274 | GenBank | ENF75972.1 | 714 | fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.10] |
GI:16130274 | GenBank | ENF85935.1 | 714 | fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.12] |
GI:16130274 | GenBank | ENF90539.1 | 714 | fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.13] |
GI:16130274 | GenBank | ENF96306.1 | 714 | fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.15] |
GI:16130274 | GenBank | ENG02945.1 | 714 | fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.3] |
GI:16130274 | GenBank | ENG12373.1 | 714 | fatty oxidation complex, alpha subunit FadJ [Escherichia coli P0305260.5] |