Gene/Proteome Database (LMPD)

LMPD ID
LMP007647
Gene ID
946991
Species
Escherichia coli K-12 (E. coli)
Gene Name
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
Gene Symbol
ispG
Synonyms
ECK2511; JW2499; gcpE
Summary
The IspG substrate is 2C-methyl-d-erythritol 2,4-cyclodiphosphate. [More information is available at EcoGene: EG10370]. 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase (IspG) catalyzes the sixth step in the methylerythritol phosphate pathway. [More information is available at EcoCyc: EG10370].
Orthologs
View orthologs and multiple alignments for ispG

Proteins

1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_417010
Protein GI 16130440
UniProt ID P62620
Length 372
RefSeq Status REVIEWED
MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEATVNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLESAMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGVRKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK

Gene Information

Entrez Gene ID
946991
Gene Name
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
Gene Symbol
ispG
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0051539 IDA:EcoCyc F 4 iron, 4 sulfur cluster binding
GO:0046429 IDA:EcoCyc F 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
GO:0005506 IEA:InterPro F iron ion binding
GO:0046872 IDA:EcoCyc F metal ion binding
GO:0019288 IEA:UniProtKB-UniPathway P isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
GO:0016114 IEA:UniProtKB-HAMAP P terpenoid biosynthetic process
GO:0006744 IDA:EcoCyc P ubiquinone biosynthetic process

KEGG Pathway Links

KEGG Pathway ID Description
eco01110 Biosynthesis of secondary metabolites
M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco_M00096 C5 isoprenoid biosynthesis, non-mevalonate pathway
eco01100 Metabolic pathways
eco00900 Terpenoid backbone biosynthesis
ko00900 Terpenoid backbone biosynthesis

BIOCYC Pathway Links

BIOCYC Pathway ID Description
NONMEVIPP-PWY methylerythritol phosphate pathway
NONMEVIPP-PWY methylerythritol phosphate pathway
PWY-5121 superpathway of geranylgeranyl diphosphate biosynthesis II (via MEP)
PWY-7392 taxadiene biosynthesis (engineered)

Domain Information

InterPro Annotations

Accession Description
IPR013785 Aldolase-type TIM barrel
IPR011005 Dihydropteroate_synth-like
IPR016425 IspG_bac
IPR004588 IspG_bac-typ

UniProt Annotations

Entry Information

Gene Name
1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
Protein Entry
ISPG_ECOLI
UniProt ID
P62620
Species
E. coli

Comments

Comment Type Description
Catalytic Activity (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + H(2)O + 2 oxidized ferredoxin = 2-C-methyl-D- erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin. {ECO:0000269|PubMed:12571359}.
Cofactor Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
Function Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate.
Interaction P77188:ecpB; NbExp=2; IntAct=EBI-550338, EBI-1113651;
Pathway Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1- deoxy-D-xylulose 5-phosphate: step 5/6. {ECO:0000269|PubMed:11163766, ECO:0000269|PubMed:11274098}.
Similarity Belongs to the IspG family. {ECO:0000305}.

Identical and Related Proteins

Unique RefSeq proteins for LMP007647 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16130440 RefSeq NP_417010 372 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007647 proteins

Reference Database Accession Length Protein Name
GI:16130440 GenBank KHJ01168.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli]
GI:16130440 GenBank KHJ09804.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli]
GI:16130440 GenBank KHJ14056.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli]
GI:16130440 GenBank KHJ21446.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli]
GI:16130440 GenBank KHJ27161.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli]
GI:16130440 gnl IGS 372 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [Escherichia coli ER2796]

Related Sequences to LMP007647 proteins

Reference Database Accession Length Protein Name
GI:16130440 DBBJ GAL54916.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia albertii NBRC 107761]
GI:16130440 GenBank EDS92860.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia albertii TW07627]
GI:16130440 GenBank EHO00727.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli E101]
GI:16130440 GenBank KDA57207.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli 2-011-08_S1_C1]
GI:16130440 RefSeq WP_000551802.1 372 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia coli]
GI:16130440 RefSeq WP_000551806.1 372 MULTISPECIES: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase [Escherichia]