Gene/Proteome Database (LMPD)

LMPD ID
LMP007740
Gene ID
Species
Escherichia coli K-12 (E. coli)
Gene Name
glycerol kinase
Gene Symbol
Synonyms
ECK3918; JW3897
Summary
The enzyme can undergo a reversible subunit dissociation between tetramer and dimer . [More information is available at EcoCyc: EG10398].
Orthologs

Proteins

glycerol kinase [Escherichia coli str. K-12 substr. MG1655]
Refseq ID NP_418361
Protein GI 16131764
UniProt ID P0A6F3
Length 502
RefSeq Status REVIEWED
MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE

Gene Information

Entrez Gene ID
Gene Name
glycerol kinase
Gene Symbol
Species
Escherichia coli K-12

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005829 IDA:UniProtKB C cytosol
GO:0005524 IEA:UniProtKB-HAMAP F ATP binding
GO:0004370 IDA:UniProtKB F glycerol kinase activity
GO:0046872 IDA:EcoCyc F metal ion binding
GO:0008270 IDA:UniProtKB F zinc ion binding
GO:0006974 IEP:EcoliWiki P cellular response to DNA damage stimulus
GO:0019563 IEA:UniProtKB-UniPathway P glycerol catabolic process
GO:0006071 IDA:UniProtKB P glycerol metabolic process
GO:0006072 IEA:UniProtKB-HAMAP P glycerol-3-phosphate metabolic process
GO:0016310 IDA:EcoCyc P phosphorylation

KEGG Pathway Links

KEGG Pathway ID Description
eco00561 Glycerolipid metabolism
ko00561 Glycerolipid metabolism
eco01100 Metabolic pathways

BIOCYC Pathway Links

BIOCYC Pathway ID Description
PWY0-381 glycerol and glycerophosphodiester degradation
PWY0-381 glycerol and glycerophosphodiester degradation
PWY-4261 glycerol degradation I
PWY0-381 glycerol degradation I
PWY-4261 glycerol degradation I

Domain Information

InterPro Annotations

Accession Description
IPR018485 Carbohydrate kinase, FGGY, C-terminal
IPR018484 Carbohydrate kinase, FGGY, N-terminal
IPR018483 Carbohydrate kinase, FGGY, conserved site
IPR005999 Glycerol kinase

UniProt Annotations

Entry Information

Gene Name
glycerol kinase
Protein Entry
GLPK_ECOLI
UniProt ID
Species
E. coli

Comments

Comment Type Description
Biophysicochemical Properties Kinetic parameters: KM=1.3 uM for glycerol {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=400 uM for D-glyceraldehyde {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=500 uM for dihydroxyacetone {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=3 mM for L-glyceraldehyde {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; KM=4 mM for ATP {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843}; pH dependence: Optimum pH is 9.8. {ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908, ECO:0000269|PubMed:9817843};
Catalytic Activity ATP + glycerol = ADP + sn-glycerol 3- phosphate. {ECO:0000255|HAMAP-Rule:MF_00186, ECO:0000269|PubMed:5335908}.
Enzyme Regulation Activity of this regulatory enzyme is affected by several metabolites. The non-competitive allosteric inhibition by fructose 1,6-bisphosphate (FBP) causes alterations in the quaternary structure of the enzyme. FBP inhibition requires that the enzyme exist only in a tetrameric state. Salt such as KCl reduces the affinity of the tetrameric form of the enzyme for FBP. Unphosphorylated phosphocarrier protein EIIA-Glc (III-Glc), an integral component of the bacterial phosphotransferase (PTS) system, also inhibits non-competitively and allostericaly the activity. Unlike FBP, both the dimer and the tetramer appear to be fully sensitive to enzyme EIIA-Glc inhibition. Zn(+2) greatly enhances the inhibitory potency of EIIA-Glc. Both allosteric regulatory agents is strongly pH dependent, with maximal inhibition occurring at pH 6.5. {ECO:0000269|PubMed:215195, ECO:0000269|PubMed:2985549, ECO:0000269|PubMed:31903, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5328677, ECO:0000269|PubMed:8430315, ECO:0000269|PubMed:9817843, ECO:0000269|PubMed:9843423}.
Function Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn- glycerol 3-phosphate. It also catalyzes the phosphorylation of dihydroxyacetone, L-glyceraldehyde and D-glyceraldehyde. It uses only ATP. {ECO:0000255|HAMAP-Rule:MF_00186, ECO:0000269|PubMed:2826434, ECO:0000269|PubMed:4575199, ECO:0000269|PubMed:5335908}.
Induction By L-alpha-glycerol 3-phosphate. {ECO:0000269|PubMed:2826434}.
Pathway Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. {ECO:0000255|HAMAP-Rule:MF_00186}.
Similarity Belongs to the FGGY kinase family. {ECO:0000255|HAMAP- Rule:MF_00186}.
Subunit Homotetramer and homodimer (in equilibrium). Heterodimer with EIIA-Glc (crr). Binds 1 zinc ion per glycerol kinase EIIA-Glc dimer. The zinc ion is important for dimerization. {ECO:0000255|HAMAP-Rule:MF_00186, ECO:0000269|PubMed:10090737, ECO:0000269|PubMed:17441732, ECO:0000269|PubMed:215194, ECO:0000269|PubMed:215195, ECO:0000269|PubMed:4934840, ECO:0000269|PubMed:8170944, ECO:0000269|PubMed:8430315, ECO:0000269|PubMed:9817843, ECO:0000269|PubMed:9843423}.
Web Resource Name=Worthington enzyme manual; URL="http://www.worthington-biochem.com/GKEC/";

Identical and Related Proteins

Unique RefSeq proteins for LMP007740 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
16131764 RefSeq NP_418361 502 glycerol kinase [Escherichia coli str. K-12 substr. MG1655]

Identical Sequences to LMP007740 proteins

Reference Database Accession Length Protein Name
GI:16131764 GenBank KHJ01009.1 502 glycerol kinase [Escherichia coli]
GI:16131764 GenBank KHJ03511.1 502 glycerol kinase [Escherichia coli]
GI:16131764 GenBank KHJ05209.1 502 glycerol kinase [Escherichia coli]
GI:16131764 GenBank KHJ11345.1 502 glycerol kinase [Escherichia coli]
GI:16131764 GenBank KHJ20039.1 502 glycerol kinase [Escherichia coli]
GI:16131764 gnl IGS 502 glycerol kinase [Escherichia coli ER2796]

Related Sequences to LMP007740 proteins

Reference Database Accession Length Protein Name
GI:16131764 GenBank ESA61829.1 509 glycerol kinase [Escherichia coli 110957]
GI:16131764 GenBank ESA64832.1 509 glycerol kinase [Escherichia coli 113303]
GI:16131764 GenBank ESA77371.1 509 glycerol kinase [Escherichia coli 907357]
GI:16131764 GenBank ESA90841.1 509 glycerol kinase [Escherichia coli 907713]
GI:16131764 GenBank ESC92625.1 509 glycerol kinase [Escherichia coli 113302]
GI:16131764 RefSeq NP_290555.1 509 glycerol kinase [Escherichia coli O157:H7 str. EDL933]