Gene/Proteome Database (LMPD)

LMPD ID
LMP010618
Gene ID
838362
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
methylcrotonoyl-CoA carboxylase subunit alpha
Gene Symbol
MCCA
Alternate Names
methylcrotonoyl-CoA carboxylase subunit alpha
Chromosome
1
EC Number
6.4.1.4
Summary
MCCA is the biotinylated subunit of the dimer MCCase, which is involved in leucine degradation. Both subunits are nuclear coded and the active enzyme is located in the mitochondrion.
Orthologs
View orthologs and multiple alignments for MCCA

Proteins

methylcrotonoyl-CoA carboxylase subunit alpha
Refseq ID NP_563674
Protein GI 18379053
UniProt ID Q42523
mRNA ID NM_100191
Length 714
RefSeq Status REVIEWED
MSMMTVWALRRNVRRKNHSMLVRYISGSASMKPKEQCIEKILVANRGEIACRIMRTAKRLGIQTVAVYSDADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFAQLCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIGYPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQIFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAAVGYYNAGTVEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSGHAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVVWGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFADESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGKVPSIWYSNPPFRVHHEAKQTIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEGNDSPSLELRVTRAGKCDFRVEAAGLSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSETSSHPPGTIVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDGSALFRIKG
methylcrotonoyl-CoA carboxylase subunit alpha
Refseq ID NP_849583
Protein GI 30678473
UniProt ID Q42523
mRNA ID NM_179252
Length 734
RefSeq Status REVIEWED
MSMMTVWALRRNVRRKNHSMLVRYISGSASMKPKEQCIEKILVANRGEIACRIMRTAKRLGIQTVAVYSDADRDSLHVKSADEAVRIGPPSARLSYLSGVTIMEAAARTGAQAIHPGYGFLSESSDFAQLCEDSGLTFIGPPASAIRDMGDKSASKRIMGAAGVPLVPGYHGHEQDIDHMKSEAEKIGYPIIIKPTHGGGGKGMRIVQSGKDFADSFLGAQREAAASFGVNTILLEKYITRPRHIEVQIFGDKHGNVLHLYERDCSVQRRHQKIIEEAPAPNISEKFRANLGQAAVSAARAVGYYNAGTVEFIVDTESDQFYFMEMNTRLQVEHPVTEMIVGQDLVEWQIRVANGEPLPLSQSEVPMSGHAFEARIYAENVPKGFLPATGVLNHYRPVAVSPSVRVETGVEQGDTVSMHYDPMIAKLVVWGGNRGEALVKLKDCLSNFQVAGVPTNINFLQKLASHKEFAVGNVETHFIEHHKSDLFADESNPAATEVAYKAVKHSAALVAACISTIEHSTWNESNHGKVPSIWYSNPPFRVHHEAKQTIELEWNNECEGTGSNLISLGVRYQPDGSYLIEEGNDSPSLELRVTRAGKCDFRVEAAGLSMNVSLAAYLKDGYKHIHIWHGSEHHQFKQKVGIEFSEDEEGVQHRTSSETSSHPPGTIVAPMAGLVVKVLVENEAKVDQGQPILVLEAMKMEHVVKAPSSGSIQDLKVKAGQQVSDGSALFRIKG

Gene Information

Entrez Gene ID
838362
Gene Name
methylcrotonoyl-CoA carboxylase subunit alpha
Gene Symbol
MCCA
Species
Arabidopsis thaliana

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0022626 IDA:TAIR C cytosolic ribosome
GO:0005739 IDA:TAIR C mitochondrion
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004075 IEA:InterPro F biotin carboxylase activity
GO:0050897 IDA:TAIR F cobalt ion binding
GO:0004485 IDA:TAIR F methylcrotonoyl-CoA carboxylase activity
GO:0006552 IDA:TAIR P leucine catabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ath_M00036 Leucine degradation, leucine => acetoacetate + acetyl-CoA
ath01100 Metabolic pathways

REACTOME Pathway Links

REACTOME Pathway ID Description
6254406 Biotin transport and metabolism
6253683 Branched-chain amino acid catabolism

Domain Information

InterPro Annotations

Accession Description
IPR011761 ATP-grasp fold
IPR013815 ATP-grasp fold, subdomain 1
IPR013816 ATP-grasp fold, subdomain 2
IPR005482 Biotin carboxylase, C-terminal
IPR011764 Biotin carboxylation domain
IPR001882 Biotin-binding site
IPR000089 Biotin/lipoyl attachment
IPR005481 Carbamoyl-phosphate synthase, large subunit, N-terminal
IPR005479 Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
IPR016185 Pre-ATP-grasp domain
IPR011054 Rudiment single hybrid motif
IPR011053 Single hybrid motif

UniProt Annotations

Entry Information

Gene Name
methylcrotonoyl-CoA carboxylase subunit alpha
Protein Entry
MCCA_ARATH
UniProt ID
Q42523
Species
Arabidopsis

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; IsoId=Q42523-1; Sequence=Displayed; Name=2; IsoId=Q42523-2; Sequence=VSP_008910; Note=May be due to exon skipping. No experimental confirmation available.;
Catalytic Activity ATP + 3-methylcrotonoyl-CoA + HCO(3)(-) = ADP + phosphate + 3-methylglutaconyl-CoA.
Cofactor Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250}; Note=Binds 2 manganese ions per subunit. {ECO:0000250};
Cofactor Name=biotin; Xref=ChEBI:CHEBI:57586; Note=Biotin.;
Function Biotin-attachment subunit of the 3-methylcrotonyl-CoA carboxylase, an enzyme that catalyzes the conversion of 3- methylcrotonyl-CoA to 3-methylglutaconyl-CoA, a critical step for leucine and isovaleric acid catabolism. {ECO:0000250}.
Miscellaneous Temporal and spatial accumulation of the alpha and beta subunits during development at approximately equal molar ratios.
Pathway Amino-acid degradation; L-leucine degradation; (S)-3- hydroxy-3-methylglutaryl-CoA from 3-isovaleryl-CoA: step 2/3.
Sequence Caution Sequence=AAD25800.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
Similarity Contains 1 ATP-grasp domain. {ECO:0000255|PROSITE- ProRule:PRU00409}.
Similarity Contains 1 biotin carboxylation domain. {ECO:0000305}.
Similarity Contains 1 biotinyl-binding domain. {ECO:0000255|PROSITE-ProRule:PRU01066, ECO:0000305}.
Subcellular Location Mitochondrion matrix.
Subunit Probably a heterodimer composed of biotin-containing alpha subunits and beta subunits. {ECO:0000250}.
Tissue Specificity In roots, cotyledons, leaves, flowers, ovaries, siliques and embryos. {ECO:0000269|PubMed:10681539}.

Identical and Related Proteins

Unique RefSeq proteins for LMP010618 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
30678473 RefSeq NP_849583 734 methylcrotonoyl-CoA carboxylase subunit alpha
18379053 RefSeq NP_563674 714 methylcrotonoyl-CoA carboxylase subunit alpha

Identical Sequences to LMP010618 proteins

Reference Database Accession Length Protein Name
GI:30678473 GenBank AAL50065.1 734 At1g03090/F10O3_8 [Arabidopsis thaliana]
GI:30678473 GenBank AEE27527.1 734 methylcrotonoyl-CoA carboxylase subunit alpha [Arabidopsis thaliana]
GI:18379053 GenBank AEE27528.1 714 methylcrotonoyl-CoA carboxylase subunit alpha [Arabidopsis thaliana]
GI:30678473 SwissProt Q42523.2 734 RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor [Arabidopsis thaliana]

Related Sequences to LMP010618 proteins

Reference Database Accession Length Protein Name
GI:18379053 GenBank AAA67356.1 715 3-methylcrotonyl-CoA carboxylase precursor [Arabidopsis thaliana]
GI:30678473 GenBank AAA67356.1 715 3-methylcrotonyl-CoA carboxylase precursor [Arabidopsis thaliana]
GI:30678473 GenBank AAD25800.1 730 Identical to gb|U12536 3-methylcrotonyl-CoA carboxylase precursor protein from Arabidopsis thaliana. ESTs gb|H35836, gb|AA651295 and gb|AA721862 come from this gene [Arabidopsis thaliana]
GI:18379053 GenBank AAD25800.1 730 Identical to gb|U12536 3-methylcrotonyl-CoA carboxylase precursor protein from Arabidopsis thaliana. ESTs gb|H35836, gb|AA651295 and gb|AA721862 come from this gene [Arabidopsis thaliana]
GI:18379053 GenBank AAL50065.1 734 At1g03090/F10O3_8 [Arabidopsis thaliana]
GI:30678473 GenBank EFH65694.1 734 3-methylcrotonyl-CoA carboxylase 1 [Arabidopsis lyrata subsp. lyrata]
GI:18379053 GenBank AEE27527.1 734 methylcrotonoyl-CoA carboxylase subunit alpha [Arabidopsis thaliana]
GI:30678473 GenBank AEE27528.1 714 methylcrotonoyl-CoA carboxylase subunit alpha [Arabidopsis thaliana]
GI:30678473 RefSeq NP_563674.1 714 methylcrotonoyl-CoA carboxylase subunit alpha [Arabidopsis thaliana]
GI:18379053 RefSeq NP_849583.1 734 methylcrotonoyl-CoA carboxylase subunit alpha [Arabidopsis thaliana]
GI:30678473 RefSeq XP_002889435.1 734 3-methylcrotonyl-CoA carboxylase 1 [Arabidopsis lyrata subsp. lyrata]
GI:18379053 SwissProt Q42523.2 734 RecName: Full=Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; Short=MCCase subunit alpha; AltName: Full=3-methylcrotonyl-CoA carboxylase 1; AltName: Full=3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; Flags: Precursor [Arabidopsis thaliana]