Gene/Proteome Database (LMPD)
LMPD ID
LMP010731
Gene ID
Species
Arabidopsis thaliana (Arabidopsis)
Gene Name
phospholipase A 2A
Gene Symbol
Synonyms
PATATIN-LIKE PROTEIN 2; phospholipase A 2A; PHOSPHOLIPASE A 2A; PLA IIA; PLA2A; PLP2; T9J22.23; T9J22_23
Alternate Names
phospholipase A 2A
Chromosome
2
EC Number
3.1.1.-
Summary
Encodes a lipid acyl hydrolase with wide substrate specificity that accumulates upon infection by fungal and bacterial pathogens. Protein is localized in the cytoplasm in healthy leaves, and in membranes in infected cells. Plays a role in cell death and differentially affects the accumulation of oxylipins. Contributes to resistance to virus.
Orthologs
Proteins
| phospholipase A 2A | |
|---|---|
| Refseq ID | NP_180224 |
| Protein GI | 15225332 |
| UniProt ID | O48723 |
| mRNA ID | NM_128213 |
| Length | 407 |
| RefSeq Status | REVIEWED |
| MQMDSPKSPLQPPTYGNLVTILSIDGGGIRGLIPAVILGFLESELQKLDGEEARLADYFDVIAGTSTGGLVTAMLTAPNKEGRPLFAASEIKDFYLEQCPKIFPQDHFPFSAAKKLVKSLTGPKYDGKYLHQLIHAKLGDTKLSQTLTNVVIPTFDIKHLQPTIFSSYEVKNHPLKDATLADIAISTSAAPTYLPAHFFKVEDLNGNAKEYNLIDGGVAANNPALLAIGEVTNEISGGSSDFFPIRPNDYGRFLVLSLGTGNHKAEEKFNAKEVAGWGLLNWLTHDNSTPIIDAFSQASSDMVDFHLSAVFRALHSEANYIRIQDDTLTGDAASVDIATVENLDILAKTGDELLKKPVARVNLDSGCNENAYETTNEHALIKLAGILSKEKKIRDIRSPHAKAPIRI | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005737 | IDA:TAIR | C | cytoplasm |
| GO:0016020 | IDA:TAIR | C | membrane |
| GO:0016298 | IDA:TAIR | F | lipase activity |
| GO:0008219 | IGI:TAIR | P | cell death |
| GO:0071456 | IEP:TAIR | P | cellular response to hypoxia |
| GO:0051607 | IMP:TAIR | P | defense response to virus |
| GO:0016042 | IEA:UniProtKB-KW | P | lipid catabolic process |
| GO:0006629 | IDA:TAIR | P | lipid metabolic process |
| GO:0031408 | IMP:TAIR | P | oxylipin biosynthetic process |
| GO:0009626 | IMP:TAIR | P | plant-type hypersensitive response |
| GO:0046686 | IEP:TAIR | P | response to cadmium ion |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Disruption Phenotype | No visible phenotype under normal growth conditions, but leaves of mutant plants contain decreased levels of lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine(LPE), but increased levels of free linolenic acid, jasmonic acid and methyl jasmonate, as well as the oxylipin-biosynthetic intermediates 13-hydroperoxylinolenic acid and 12-oxophytodienoic acid. {ECO:0000269|PubMed:22259021}. |
| Domain | The nitrogen atoms of the two glycine residues in the GGXR motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage. {ECO:0000250}. |
| Function | Possesses non-specific lipolytic acyl hydrolase (LAH) activity. Catalyzes the hydrolysis of the galactolipids monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), and less efficiently the phoshpolipids phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidic acid (PA), phosphatidylserine (PS) and phosphatidylinositol (PI). Favors the release of fatty acid at the sn-1 position for PC or PE and the sn-2 position for PG, PA, PS and PI. Negatively affects disease resistance to the necrotic fungal pathogen Botrytis cinerea and the avirulent bacteria Pseudomonas syringae by promoting cell death and reducing the efficiency of the hypersensitive response, respectively. However, PLP2 contributes to resistance to cucumber mosaic virus (CMV), an obligate parasite inducing hypersensitive response. May negatively regulate oxylipin production, possibly via participating in membrane repair that includes removal of oxidatively modified lipids. {ECO:0000269|PubMed:16297072, ECO:0000269|PubMed:19271961, ECO:0000269|PubMed:22259021}. |
| Induction | By infection with the fungal pathogens B.cinerea and A.brassicicola, and avirulent and virulent strains of P.syringae pv tomato DC3000 (at protein level). Induced by ethylene and copper. {ECO:0000269|PubMed:14634163, ECO:0000269|PubMed:16297072}. |
| Sequence Caution | Sequence=AAM63157.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; |
| Similarity | Belongs to the patatin family. {ECO:0000305}. |
| Similarity | Contains 1 patatin domain. {ECO:0000305}. |
| Subcellular Location | Cytoplasm {ECO:0000269|PubMed:12226489, ECO:0000269|PubMed:16297072}. |
| Tissue Specificity | Expressed specifically in roots. {ECO:0000269|PubMed:12226489}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP010731 (as displayed in Record Overview)
Identical Sequences to LMP010731 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15225332 | GenBank | AAL32722.1 | 407 | similar to latex allergen [Arabidopsis thaliana] |
| GI:15225332 | GenBank | AAM13304.1 | 407 | similar to latex allergen [Arabidopsis thaliana] |
| GI:15225332 | GenBank | AEC07858.1 | 407 | phospholipase A 2A [Arabidopsis thaliana] |
| GI:15225332 | gnl | TIGR | 407 | similar to latex allergen from Hevea brasiliensis [Arabidopsis thaliana] |
| GI:15225332 | SwissProt | O48723.1 | 407 | RecName: Full=Patatin-like protein 2; Short=AtPLP2; AltName: Full=Patatin-related phospholipase A IIalpha; Short=pPLAIIa; AltName: Full=Phospholipase A IIA; Short=AtPLAIIA [Arabidopsis thaliana] |
Related Sequences to LMP010731 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:15225332 | GenBank | AAM63157.1 | 405 | similar to latex allergen from Hevea brasiliensis [Arabidopsis thaliana] |
| GI:15225332 | GenBank | EFH57069.1 | 407 | hypothetical protein ARALYDRAFT_481516 [Arabidopsis lyrata subsp. lyrata] |
| GI:15225332 | RefSeq | XP_002880810.1 | 407 | hypothetical protein ARALYDRAFT_481516 [Arabidopsis lyrata subsp. lyrata] |
| GI:15225332 | RefSeq | XP_010417357.1 | 407 | PREDICTED: patatin-like protein 2 [Camelina sativa] |
| GI:15225332 | RefSeq | XP_010429587.1 | 407 | PREDICTED: patatin-like protein 2 [Camelina sativa] |
| GI:15225332 | RefSeq | XP_010472600.1 | 407 | PREDICTED: patatin-like protein 2 [Camelina sativa] |