Gene/Proteome Database (LMPD)
LMPD ID
LMP012378
Gene ID
Species
Homo sapiens (Human)
Gene Name
phospholipase A2, group IVB (cytosolic)
Gene Symbol
Synonyms
HsT16992; cPLA2-beta;
Chromosome
15
Map Location
15q11.2-q21.3
EC Number
3.1.1.4
Summary
This gene encodes a member of the cytosolic phospholipase A2 protein family. Phospholipase A2 enzymes hydrolyze the sn-2 bond of phospholipids, releasing lysophospholipids and fatty acids. This enzyme may be associated with mitochondria and early endosomes. Most tissues also express read-through transcripts from the upstream gene into this gene, some of which may encode fusion proteins combining the N-terminus of the upstream gene including its JmjC domain with the almost complete coding region of this gene, including the C2 and cytoplasmic phospholipase A2 domains. [provided by RefSeq, Jul 2008]
Orthologs
Proteins
| cytosolic phospholipase A2 beta | |
|---|---|
| Refseq ID | NP_001108105 |
| Protein GI | 167860120 |
| UniProt ID | P0C869 |
| mRNA ID | NM_001114633 |
| Length | 781 |
| MAVAEVSRTCLLTVRVLQAHRLPSKDLVTPSDCYVTLWLPTACSHRLQTRTVKNSSSPVWNQSFHFRIHRQLKNVMELKVFDQDLVTGDDPVLSVLFDAGTLRAGEFRRESFSLSPQGEGRLEVEFRLQSLADRGEWLVSNGVLVARELSCLHVQLEETGDQKSSEHRVQLVVPGSCEGPQEASVGTGTFRFHCPACWEQELSIRLQDAPEEQLKAPLSALPSGQVVRLVFPTSQEPLMRVELKKEAGLRELAVRLGFGPCAEEQAFLSRRKQVVAAALRQALQLDGDLQEDEIPVVAIMATGGGIRAMTSLYGQLAGLKELGLLDCVSYITGASGSTWALANLYEDPEWSQKDLAGPTELLKTQVTKNKLGVLAPSQLQRYRQELAERARLGYPSCFTNLWALINEALLHDEPHDHKLSDQREALSHGQNPLPIYCALNTKGQSLTTFEFGEWCEFSPYEVGFPKYGAFIPSELFGSEFFMGQLMKRLPESRICFLEGIWSNLYAANLQDSLYWASEPSQFWDRWVRNQANLDKEQVPLLKIEEPPSTAGRIAEFFTDLLTWRPLAQATHNFLRGLHFHKDYFQHPHFSTWKATTLDGLPNQLTPSEPHLCLLDVGYLINTSCLPLLQPTRDVDLILSLDYNLHGAFQQLQLLGRFCQEQGIPFPPISPSPEEQLQPRECHTFSDPTCPGAPAVLHFPLVSDSFREYSAPGVRRTPEEAAAGEVNLSSSDSPYHYTKVTYSQEDVDKLLHLTHYNVCNNQEQLLEALRQAVQRRRQRRPH | |
Gene Information
Entrez Gene ID
Gene Name
phospholipase A2, group IVB (cytosolic)
Gene Symbol
Species
Homo sapiens
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0005829 | IDA:UniProtKB | C | cytosol |
| GO:0005768 | IEA:UniProtKB-KW | C | endosome |
| GO:0005576 | TAS:UniProtKB | C | extracellular region |
| GO:0005743 | TAS:Reactome | C | mitochondrial inner membrane |
| GO:0005509 | NAS:UniProtKB | F | calcium ion binding |
| GO:0047498 | IDA:UniProtKB | F | calcium-dependent phospholipase A2 activity |
| GO:0005544 | NAS:UniProtKB | F | calcium-dependent phospholipid binding |
| GO:0004622 | NAS:UniProtKB | F | lysophospholipase activity |
| GO:0006987 | TAS:Reactome | P | activation of signaling protein activity involved in unfolded protein response |
| GO:0019369 | NAS:UniProtKB | P | arachidonic acid metabolic process |
| GO:0019722 | NAS:UniProtKB | P | calcium-mediated signaling |
| GO:0044267 | TAS:Reactome | P | cellular protein metabolic process |
| GO:0030968 | TAS:Reactome | P | endoplasmic reticulum unfolded protein response |
| GO:0046474 | TAS:Reactome | P | glycerophospholipid biosynthetic process |
| GO:0046475 | IDA:UniProtKB | P | glycerophospholipid catabolic process |
| GO:0006954 | NAS:UniProtKB | P | inflammatory response |
| GO:0007567 | NAS:UniProtKB | P | parturition |
| GO:0006654 | TAS:Reactome | P | phosphatidic acid biosynthetic process |
| GO:0036151 | TAS:Reactome | P | phosphatidylcholine acyl-chain remodeling |
| GO:0036152 | TAS:Reactome | P | phosphatidylethanolamine acyl-chain remodeling |
| GO:0036148 | TAS:Reactome | P | phosphatidylglycerol acyl-chain remodeling |
| GO:0036150 | TAS:Reactome | P | phosphatidylserine acyl-chain remodeling |
| GO:0006644 | TAS:Reactome | P | phospholipid metabolic process |
| GO:0044281 | TAS:Reactome | P | small molecule metabolic process |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| hsa00590 | Arachidonic acid metabolism |
| ko00590 | Arachidonic acid metabolism |
| hsa00565 | Ether lipid metabolism |
| ko00565 | Ether lipid metabolism |
| hsa04664 | Fc epsilon RI signaling pathway |
| ko04664 | Fc epsilon RI signaling pathway |
| hsa04666 | Fc gamma R-mediated phagocytosis |
| ko04666 | Fc gamma R-mediated phagocytosis |
| hsa04724 | Glutamatergic synapse |
| ko04724 | Glutamatergic synapse |
| hsa00564 | Glycerophospholipid metabolism |
| ko00564 | Glycerophospholipid metabolism |
| hsa04912 | GnRH signaling pathway |
| ko04912 | GnRH signaling pathway |
| hsa04750 | Inflammatory mediator regulation of TRP channels |
| ko04750 | Inflammatory mediator regulation of TRP channels |
| hsa00591 | Linoleic acid metabolism |
| ko00591 | Linoleic acid metabolism |
| hsa04730 | Long-term depression |
| ko04730 | Long-term depression |
| hsa04010 | MAPK signaling pathway |
| ko04010 | MAPK signaling pathway |
| hsa01100 | Metabolic pathways |
| hsa04913 | Ovarian steroidogenesis |
| ko04913 | Ovarian steroidogenesis |
| hsa04921 | Oxytocin signaling pathway |
| ko04921 | Oxytocin signaling pathway |
| hsa04611 | Platelet activation |
| hsa04014 | Ras signaling pathway |
| hsa04726 | Serotonergic synapse |
| hsa04370 | VEGF signaling pathway |
| ko04370 | VEGF signaling pathway |
| hsa04270 | Vascular smooth muscle contraction |
| ko04270 | Vascular smooth muscle contraction |
| hsa00592 | alpha-Linolenic acid metabolism |
| ko00592 | alpha-Linolenic acid metabolism |
REACTOME Pathway Links
| REACTOME Pathway ID | Description |
|---|---|
| REACT_120829 | Acyl chain remodelling of PC |
| REACT_121369 | Acyl chain remodelling of PE |
| REACT_121324 | Acyl chain remodelling of PG |
| REACT_121384 | Acyl chain remodelling of PS |
| REACT_121401 | Glycerophospholipid biosynthesis |
| REACT_120977 | Hydrolysis of LPC |
| REACT_18368 | IRE1alpha activates chaperones |
| REACT_111217 | Metabolism |
| REACT_22258 | Metabolism of lipids and lipoproteins |
| REACT_17015 | Metabolism of proteins |
| REACT_120870 | Phospholipid metabolism |
| REACT_120906 | Synthesis of PA |
| REACT_18356 | Unfolded Protein Response (UPR) |
| REACT_18273 | XBP1(S) activates chaperone genes |
Domain Information
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Alternative Products | Event=Alternative splicing; Named isoforms=5; Name=1; IsoId=P0C869-1, O95712-1; Sequence=Displayed; Name=2; Synonyms=beta2; IsoId=P0C869-7; Sequence=VSP_039387, VSP_039389, VSP_039390; Note=Based on a naturally occurring readthrough transcript which produces a JMJD7-PLA2G4B fusion protein.; Name=3; Synonyms=beta3; IsoId=P0C869-8; Sequence=VSP_039387, VSP_039388; Note=Based on a naturally occurring readthrough transcript which produces a JMJD7-PLA2G4B fusion protein.; Name=4; IsoId=P0C869-4, O95712-4; Sequence=VSP_019871; Note=No experimental confirmation available.; Name=5; Synonyms=Beta1; IsoId=P0C869-6; Sequence=VSP_039387; Note=Based on a naturally occurring readthrough transcript which produces a JMJD7-PLA2G4B fusion protein.; |
| Biophysicochemical Properties | Kinetic parameters: Vmax=7.9 pmol/min/mg enzyme (isoform 5) ; Vmax=3.6 pmol/min/ug enzyme with cAMP as substrate (isoform 4) ; Vmax=140 nmol/min/ug enzyme with 1-[14C]16:0-2-lyso-PC as substrate (isoform 5) ; Vmax=1.6 nmol/min/ug enzyme with 1-[14C]16:0-2-lyso-PC as substrate (isoform 3) ; Vmax=2.1 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PC as substrate (isoform 5) ; Vmax=0.6 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PE as substrate (isoform 5) ; Vmax=0.8 nmol/min/ug enzyme with 1-16:0-2-[14C]20:4-PE as substrate (isoform 3) ; Vmax=0.3 nmol/min/ug enzyme with 1-16:0-2-[14C]18:2-PE as substrate (isoform 3) ; |
| Catalytic Activity | Phosphatidylcholine + H(2)O = 1- acylglycerophosphocholine + a carboxylate. {ECO:0000269|PubMed:10085124, ECO:0000269|PubMed:10358058, ECO:0000269|PubMed:16617059}. |
| Caution | Most tissues also express read-through transcripts from this gene into the upstream gene (JMJD7), some of which may encode fusion proteins |
| Domain | The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+) (By similarity) |
| Enzyme Regulation | Stimulated by cytosolic Ca(2+) |
| Function | Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position with a preference for arachidonoyl phospholipids. Has a much weaker activity than PLA2G4A. Isoform 3 has calcium-dependent activity against palmitoyl-arachidonyl-phosphatidylethanolamine and low level lysophospholipase activity but no activity against phosphatidylcholine. Isoform 5 does have activity against phosphatidylcholine. {ECO:0000269|PubMed:10085124, ECO:0000269|PubMed:10358058, ECO:0000269|PubMed:16617059}. |
| Sequence Caution | Sequence=BAD92387.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence= ; |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 PLA2c domain. {ECO:0000255|PROSITE- ProRule:PRU00555}. |
| Subcellular Location | Cytoplasm, cytosol |
| Subcellular Location | Isoform 3: Mitochondrion membrane; Peripheral membrane protein. Early endosome membrane; Peripheral membrane protein. Note=Translocates to membrane vesicles in a calcium-dependent fashion. |
| Tissue Specificity | Widely expressed. Expressed at higher level in brain, heart, liver, cerebellum and pancreas. Isoform 3 is widely expressed. {ECO:0000269|PubMed:10085124, ECO:0000269|PubMed:10358058, ECO:0000269|PubMed:16617059}. |
Identical and Related Proteins
Unique RefSeq proteins for LMP012378 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 167860120 | RefSeq | NP_001108105 | 781 | cytosolic phospholipase A2 beta |
Identical Sequences to LMP012378 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|
Related Sequences to LMP012378 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|