Gene/Proteome Database (LMPD)
LMPD ID
LMP012556
Gene ID
Species
Rattus norvegicus (Rat)
Gene Name
phospholipase C, gamma 1
Gene Symbol
Synonyms
PPLCA;
Alternate Names
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; PLC-II; PLC-148; PLC-gamma-1; phospholipase C-gamma-1; phosphoinositide phospholipase C; phosphoinositide phospholipase C-gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1;
Chromosome
3
Map Location
3q42
EC Number
3.1.4.11
Summary
mediates intracellular agonist-induced Ca2+ entry (ACE); serves as a guanine nucleotide exchange factor for the nuclear GTPase PIKE [RGD, Feb 2006]
Orthologs
Proteins
| 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 | |
|---|---|
| Refseq ID | NP_037319 |
| Protein GI | 6981370 |
| UniProt ID | P10686 |
| mRNA ID | NM_013187 |
| Length | 1290 |
| MAGVGTPCANGCGPSAPSEAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGSIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAATPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDFEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLETNTLRTGERPELCQVSLSEFQQFLLEYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDELVTFLFSKENSVWNSQLDAVCPETMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQHFRKVLGDTLLTKPVDIAADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSVMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEASGSTELHSSEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLWFPSNYVEEMINPAILEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMPSVAQWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERASDASSQLFHVRAREGSFEARYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL | |
Gene Information
Gene Ontology (GO Annotations)
| GO ID | Source | Type | Description |
|---|---|---|---|
| GO:0008180 | ISS:UniProtKB | C | COP9 signalosome |
| GO:0005737 | TAS:RGD | C | cytoplasm |
| GO:0030027 | ISS:UniProtKB | C | lamellipodium |
| GO:0005886 | TAS:Reactome | C | plasma membrane |
| GO:0001726 | ISS:UniProtKB | C | ruffle |
| GO:0005509 | IEA:InterPro | F | calcium ion binding |
| GO:0005158 | IDA:RGD | F | insulin receptor binding |
| GO:0004435 | IEA:UniProtKB-EC | F | phosphatidylinositol phospholipase C activity |
| GO:0051219 | IPI:RGD | F | phosphoprotein binding |
| GO:0004871 | IEA:UniProtKB-KW | F | signal transducer activity |
| GO:0006816 | IMP:RGD | P | calcium ion transport |
| GO:0071364 | ISS:UniProtKB | P | cellular response to epidermal growth factor stimulus |
| GO:0032959 | IMP:RGD | P | inositol trisphosphate biosynthetic process |
| GO:0035556 | IEA:InterPro | P | intracellular signal transduction |
| GO:0009395 | IEA:InterPro | P | phospholipid catabolic process |
| GO:0010634 | IMP:RGD | P | positive regulation of epithelial cell migration |
| GO:0009306 | IMP:RGD | P | protein secretion |
| GO:1901339 | IMP:RGD | P | regulation of store-operated calcium channel activity |
| GO:0009629 | IEP:RGD | P | response to gravity |
| GO:0042542 | IEP:RGD | P | response to hydrogen peroxide |
| GO:0043278 | IEP:RGD | P | response to morphine |
| GO:0010243 | IEP:RGD | P | response to organonitrogen compound |
| GO:0007165 | TAS:RGD | P | signal transduction |
KEGG Pathway Links
| KEGG Pathway ID | Description |
|---|---|
| ko04020 | Calcium signaling pathway |
| rno04020 | Calcium signaling pathway |
| ko05169 | Epstein-Barr virus infection |
| rno05169 | Epstein-Barr virus infection |
| ko04012 | ErbB signaling pathway |
| rno04012 | ErbB signaling pathway |
| ko04664 | Fc epsilon RI signaling pathway |
| rno04664 | Fc epsilon RI signaling pathway |
| ko04666 | Fc gamma R-mediated phagocytosis |
| rno04666 | Fc gamma R-mediated phagocytosis |
| ko05214 | Glioma |
| rno05214 | Glioma |
| rno04066 | HIF-1 signaling pathway |
| ko04750 | Inflammatory mediator regulation of TRP channels |
| rno04750 | Inflammatory mediator regulation of TRP channels |
| ko00562 | Inositol phosphate metabolism |
| rno00562 | Inositol phosphate metabolism |
| M00130 | Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 |
| ko04670 | Leukocyte transendothelial migration |
| rno04670 | Leukocyte transendothelial migration |
| rno01100 | Metabolic pathways |
| ko05206 | MicroRNAs in cancer |
| rno05206 | MicroRNAs in cancer |
| rno04064 | NF-kappa B signaling pathway |
| ko04650 | Natural killer cell mediated cytotoxicity |
| rno04650 | Natural killer cell mediated cytotoxicity |
| ko04722 | Neurotrophin signaling pathway |
| rno04722 | Neurotrophin signaling pathway |
| ko05223 | Non-small cell lung cancer |
| rno05223 | Non-small cell lung cancer |
| rno05200 | Pathways in cancer |
| ko04070 | Phosphatidylinositol signaling system |
| rno04070 | Phosphatidylinositol signaling system |
| ko05205 | Proteoglycans in cancer |
| rno05205 | Proteoglycans in cancer |
| ko04015 | Rap1 signaling pathway |
| rno04015 | Rap1 signaling pathway |
| rno04014 | Ras signaling pathway |
| ko04660 | T cell receptor signaling pathway |
| rno04660 | T cell receptor signaling pathway |
| rno04919 | Thyroid hormone signaling pathway |
| ko04370 | VEGF signaling pathway |
| rno04370 | VEGF signaling pathway |
Domain Information
InterPro Annotations
| Accession | Description |
|---|---|
| IPR028380 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 |
| IPR000008 | C2 domain |
| IPR018247 | EF-Hand 1, calcium-binding site |
| IPR002048 | EF-hand domain |
| IPR011992 | EF-hand domain pair |
| IPR017946 | PLC-like phosphodiesterase, TIM beta/alpha-barrel domain |
| IPR016279 | Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma |
| IPR001192 | Phosphoinositide phospholipase C family |
| IPR000909 | Phospholipase C, phosphatidylinositol-specific , X domain |
| IPR001711 | Phospholipase C, phosphatidylinositol-specific, Y domain |
| IPR015359 | Phospholipase C, phosphoinositol-specific, EF-hand-like |
| IPR001849 | Pleckstrin homology domain |
| IPR011993 | Pleckstrin homology-like domain |
| IPR000980 | SH2 domain |
| IPR001452 | SH3_domain |
UniProt Annotations
Entry Information
Comments
| Comment Type | Description |
|---|---|
| Catalytic Activity | 1-phosphatidyl-1D-myo-inositol 4,5- bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol. |
| Cofactor | Name=Ca(2+); Xref=ChEBI:CHEBI:29108; |
| Domain | The SH3 domain mediates interaction with CLNK and RALGPS1 |
| Enzyme Regulation | Activated by phosphorylation on tyrosine residues |
| Function | Mediates the production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Plays an important role in the regulation of intracellular signaling cascades. Becomes activated in response to ligand- mediated activation of receptor-type tyrosine kinases, such as PDGFRA, PDGFRB, FGFR1, FGFR2, FGFR3 and FGFR4. Plays a role in actin reorganization and cell migration (By similarity) |
| Interaction | P11362:FGFR1 (xeno); NbExp=4; IntAct=EBI-520788, EBI-1028277; Q04589:Fgfr1; NbExp=2; IntAct=EBI-520788, EBI-2480918; Q62120:Jak2 (xeno); NbExp=3; IntAct=EBI-520788, EBI-646604; P18031:PTPN1 (xeno); NbExp=4; IntAct=EBI-520788, EBI-968788; Q13507:TRPC3 (xeno); NbExp=2; IntAct=EBI-520788, EBI-520807; |
| Ptm | May be dephosphorylated by PTPRJ (By similarity). Tyrosine phosphorylated by activated FGFR1, FGFR2, FGFR3 and FGFR4. The receptor-mediated activation of PLCG1 involves its phosphorylation by tyrosine kinases in response to ligation of a variety of growth factor receptors and immune system receptors. For instance, SYK phosphorylates and activates PLCG1 in response to ligation of the B-cell receptor. Tyrosine phosphorylated in response to signaling via activated FLT1, FLT3, KIT and PDGFRB. Phosphorylated by ITK and TXK on Tyr-783 upon TCR activation in T-cells (By similarity) |
| Ptm | Ubiquitinated by CBLB in activated T-cells |
| Similarity | Contains 1 C2 domain. {ECO:0000255|PROSITE- ProRule:PRU00041}. |
| Similarity | Contains 1 EF-hand domain. {ECO:0000255|PROSITE- ProRule:PRU00448}. |
| Similarity | Contains 1 PI-PLC X-box domain. {ECO:0000255|PROSITE- ProRule:PRU00270}. |
| Similarity | Contains 1 PI-PLC Y-box domain. {ECO:0000255|PROSITE- ProRule:PRU00271}. |
| Similarity | Contains 1 SH3 domain. {ECO:0000255|PROSITE- ProRule:PRU00192}. |
| Similarity | Contains 2 PH domains. {ECO:0000255|PROSITE- ProRule:PRU00145}. |
| Similarity | Contains 2 SH2 domains. {ECO:0000255|PROSITE- ProRule:PRU00191}. |
| Subcellular Location | Cell projection, lamellipodium {ECO:0000250}. Cell projection, ruffle . Note=Rapidly redistributed to ruffles and lamellipodia structures in response to epidermal growth factor (EGF) treatment |
| Subunit | Interacts (via SH2 domain) with FGFR1, FGFR2, FGFR3 and FGFR4 (phosphorylated). Interacts (via SH3 domain) with AGAP2. Interacts with LAT (phosphorylated) upon TCR activation. Interacts (via SH3 domain) with the Pro-rich domain of TNK1. Associates with BLNK, VAV1, GRB2 and NCK1 in a B-cell antigen receptor-dependent fashion. Interacts with CBLB in activated T-cells; which inhibits phosphorylation. Interacts with SHB. Interacts (via SH3 domain) with the Arg/Gly-rich-flanked Pro-rich domains of KHDRBS1/SAM68. This interaction is selectively regulated by arginine methylation of KHDRBS1/SAM68. Interacts with INPP5D/SHIP1, THEMIS and CLNK (By similarity). Interacts with RALGPS1. Interacts (via the SH2 domains) with VIL1 (phosphorylated at C-terminus tyrosine phosphorylation sites). Interacts (via SH2 domain) with RET (By similarity). Interacts with FLT1 (tyrosine-phosphorylated). Interacts with AXL, FLT4 and KIT. Interacts (via SH2 domain) with PDGFRA and PDGFRB (tyrosine phosphorylated) (By similarity). Interacts with PIP5K1C (By similarity). Interacts with NTRK1 and NTRK2 (phosphorylated upon ligand-binding) (By similarity). Interacts with SYK; activates PLCG1 (By similarity). Interacts with TESPA1 (By similarity). Interacts with GRB2, LAT and THEMIS upon TCR activation in thymocytes (By similarity) |
Identical and Related Proteins
Unique RefSeq proteins for LMP012556 (as displayed in Record Overview)
| Protein GI | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| 6981370 | RefSeq | NP_037319 | 1290 | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 |
Identical Sequences to LMP012556 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6981370 | GenBank | AAA41921.1 | 1290 | phospholipase C [Rattus norvegicus] |
| GI:6981370 | SwissProt | P10686.1 | 1290 | RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; AltName: Full=Phosphoinositide phospholipase C-gamma-1; AltName: Full=Phospholipase C-gamma-1; Short=PLC-gamma-1 [Rattus norvegicus] |
Related Sequences to LMP012556 proteins
| Reference | Database | Accession | Length | Protein Name |
|---|---|---|---|---|
| GI:6981370 | GenBank | AAA41921.1 | 1290 | phospholipase C [Rattus norvegicus] |
| GI:6981370 | GenBank | EDL96615.1 | 1290 | phospholipase C, gamma 1, isoform CRA_b [Rattus norvegicus] |
| GI:6981370 | SwissProt | P10686.1 | 1290 | RecName: Full=1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; AltName: Full=Phosphoinositide phospholipase C-gamma-1; AltName: Full=Phospholipase C-gamma-1; Short=PLC-gamma-1 [Rattus norvegicus] |