Gene/Proteome Database (LMPD)

LMPD ID
LMP012763
Gene ID
81681
Species
Rattus norvegicus (Rat)
Gene Name
lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Gene Symbol
Lss
Alternate Names
lanosterol synthase; OSC; oxidosqualene--lanosterol cyclase; 2,3-epoxysqualene--lanosterol cyclase; 2,3-oxidosqualene: lanosterol cyclase;
Chromosome
20
Map Location
20p12
EC Number
5.4.99.7
Summary
enzyme that catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Lss

Proteins

lanosterol synthase
Refseq ID NP_112311
Protein GI 13591981
UniProt ID P48450
mRNA ID NM_031049
Length 733
MTEGTCLRRRGGPYKTEPATDLTRWRLHNELGRQRWTYYQAEEDPGREQTGLEAHSLGLDTTSYFKNLPKAQTAHEGALNGVTFYAKLQAEDGHWAGDYGGPLFLLPGLLITCHIAHIPLPAGYREEMVRYLRSVQLPNGGWGLHIEDKSTVFGTALSYVSLRILGIGPDDPDLVRARNILHKKGGAVAIPSWGKFWLAVLNVYSWEGINTLFPEMWLLPEWFPAHPSTLWCHCRQVYLPMSYCYATRLSASEDPLVQSLRQELYVEDYASIDWPAQKNNVCPDDMYTPHSWLLHVVYGLLNLYERFHSTSLRKWAIQLLYEHVAADDRFTKCISIGPISKTVNMLIRWSVDGPSSPAFQEHVSRIKDYLWLGLDGMKMQGTNGSQTWDTSFAVQALLEAGAHRRPEFLPCLQKAHEFLRLSQVPDNNPDYQKYYRHMHKGGFPFSTLDCGWIVADCTAEALKAVLLLQERCPSITEHVPRERLYDAVAVLLSMRNSDGGFATYETKRGGYLLELLNPSEVFGDIMIDYTYVECTSAVMQALRHFREYFPDHRATEIRETLNQGLDFCRKKQRADGSWEGSWGVCFTYGTWFGLEAFACMGHIYQNRTACAEVAQACHFLLSRQMADGGWGEDFESCEQRRYVQSAGSQVHSTCWALLGLMAVRHPDISAQERGIRCLLGKQLPNGDWPQENISGVFNKSCAISYTNYRNIFPIWALGRFSSLYPDNTLAGHI

Gene Information

Entrez Gene ID
81681
Gene Name
lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Gene Symbol
Lss
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0043231 IDA:RGD C intracellular membrane-bounded organelle
GO:0016020 IEA:UniProtKB-KW C membrane
GO:0000250 IDA:RGD F lanosterol synthase activity
GO:0006695 IDA:RGD P cholesterol biosynthetic process
GO:0016125 IMP:RGD P sterol metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
M00101 Cholesterol biosynthesis, squalene 2,3-epoxide => cholesterol
rno01100 Metabolic pathways
ko00100 Steroid biosynthesis
rno00100 Steroid biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR001330 Prenyltransferase/squalene oxidase
IPR018333 Squalene cyclase
IPR002365 Terpene synthase, conserved site
IPR008930 Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid

UniProt Annotations

Entry Information

Gene Name
lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
Protein Entry
ERG7_RAT
UniProt ID
P48450
Species
Rat

Comments

Comment Type Description
Catalytic Activity (3S)-2,3-epoxy-2,3-dihydrosqualene = lanosterol
Disruption Phenotype Hypomorphic mutations in this gene and the Fdft1 gene were identified, as well as a null mutation in this gene. Cataract onset is associated with the specific combination of Lss and Fdft1 mutant alleles that decrease cholesterol levels in cataractous lenses to about 57% of normal. Cholesterol insufficiency may cause the deficient proliferation of lens epithelial cells in Shumiya cataract rats, resulting in the loss of homeostatic epithelial cell control of the underlying fiber cells and ultimately cataractogenesis
Function Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus
Pathway Terpene metabolism; lanosterol biosynthesis; lanosterol from farnesyl diphosphate: step 3/3.
Similarity Belongs to the terpene cyclase/mutase family
Similarity Contains 4 PFTB repeats
Subcellular Location Endoplasmic reticulum membrane {ECO:0000250}; Peripheral membrane protein .
Subunit Monomer

Identical and Related Proteins

Unique RefSeq proteins for LMP012763 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
13591981 RefSeq NP_112311 733 lanosterol synthase

Identical Sequences to LMP012763 proteins

Reference Database Accession Length Protein Name
GI:13591981 EMBL CBV30975.1 733 unnamed protein product [Rattus norvegicus]
GI:13591981 EMBL CBV31068.1 733 unnamed protein product [Rattus norvegicus]
GI:13591981 GenBank AGY09230.1 733 Sequence 98582 from patent US 8541208
GI:13591981 GenBank AGY09336.1 733 Sequence 98785 from patent US 8541208

Related Sequences to LMP012763 proteins

Reference Database Accession Length Protein Name
GI:13591981 EMBL CBG17621.1 733 unnamed protein product [Rattus norvegicus]
GI:13591981 EMBL CBG17713.1 733 unnamed protein product [Rattus norvegicus]
GI:13591981 GenBank AAA91023.1 733 oxidosqualene cyclase [Rattus norvegicus]