Gene/Proteome Database (LMPD)

LMPD ID
LMP012893
Gene ID
117243
Species
Rattus norvegicus (Rat)
Gene Name
acyl-CoA synthetase long-chain family member 6
Gene Symbol
Acsl6
Synonyms
Facl6; Lacsl;
Alternate Names
long-chain-fatty-acid--CoA ligase 6; LACS 6; long-chain acyl-CoA synthetase 6; fatty acid Coenzyme A ligase, long chain 6; long-chain-fatty-acid--CoA ligase, brain isozyme;
Chromosome
10
Map Location
10q22
EC Number
6.2.1.3
Summary
catalyzes the ligation of long chain fatty acids with coenzyme A to produce long chain acyl-CoAs in fatty acid metabolism [RGD, Feb 2006]
Orthologs
View orthologs and multiple alignments for Acsl6

Proteins

long-chain-fatty-acid--CoA ligase 6
Refseq ID NP_570095
Protein GI 18543341
UniProt ID P33124
mRNA ID NM_130739
Length 697
MQTQEILRILRLPELSDLGQFFRSLSATTLVSMGALAAILAYWLTHRPKALQPPCNLLMQSEEVEDSGGARRSVIGDCTQLLTHYYDDARTMYQVFRRGLSISGNGPCLGFRKPEQPYQWLSYQEVAKRAEFLGSGLLQHDCKVGTEQFIGVFAQNRPEWIIAELACYTYSMVVVPLYDTLGPGSIRYIINTADICTVIVDKPHKAILLLEHVERKETPGLKLVILMEPFDDALRERGKKCGVDIKSMQAIEDSGQENHRVPVPPRPDDLSIVCFTSGTTGNPKGAMLTHGNVVADFSGFLKVTESQWAPTCADVHFSYLPLAHMFERMVQSVVYCHGGRVGFFQGDIRLLSDDMKALRPTIFPVVPRLLNRMYDKIFHQADTSLKRWLLEFAAKRKQAEVRSGIIRNNSIWDELFFNKIQASLGGHVRMIVTGAAPASPTVLGFLRAALGCQVYEGYGQTECTAGCTFTTPGDWTSGHVGAPLPCNHIKLVDAEELNYWTSKGEGEICVKGPNVFKGYLKDEDRTKEALDSDGWLHTGDIGKWLPEGTLKIIDRKKDIFKLAQGEYVAPEKIENIYIRSEPVAQIYVHGDSLKAFLVGIVVPDPEVMPCWAQKKGIEGNYQELCKSKELKKAILDDMVMLGKESGLHSFEQVKAIYIHCDMFSVQNGLLTPTLKAKRPELREYFKKQIEELYSISM

Gene Information

Entrez Gene ID
117243
Gene Name
acyl-CoA synthetase long-chain family member 6
Gene Symbol
Acsl6
Species
Rattus norvegicus

Gene Ontology (GO Annotations)

GO ID Source Type Description
GO:0005783 IEA:UniProtKB-KW C endoplasmic reticulum
GO:0016021 IEA:UniProtKB-KW C integral component of membrane
GO:0005741 IEA:UniProtKB-KW C mitochondrial outer membrane
GO:0005634 IDA:RGD C nucleus
GO:0005777 IEA:UniProtKB-KW C peroxisome
GO:0005524 IEA:UniProtKB-KW F ATP binding
GO:0004467 IDA:RGD F long-chain fatty acid-CoA ligase activity
GO:0042803 IDA:UniProtKB F protein homodimerization activity
GO:0032869 IEP:RGD P cellular response to insulin stimulus
GO:0015908 IGI:RGD P fatty acid transport
GO:0001676 IDA:RGD P long-chain fatty acid metabolic process
GO:0007405 IEA:Ensembl P neuroblast proliferation
GO:0048666 IEP:RGD P neuron development
GO:0008654 IMP:RGD P phospholipid biosynthetic process
GO:0010976 IMP:RGD P positive regulation of neuron projection development
GO:0010747 IMP:RGD P positive regulation of plasma membrane long-chain fatty acid transport
GO:0010867 IGI:RGD P positive regulation of triglyceride biosynthetic process
GO:0009629 IEP:RGD P response to gravity
GO:0001666 IEP:RGD P response to hypoxia
GO:0007584 IEP:RGD P response to nutrient
GO:0048545 IEP:RGD P response to steroid hormone
GO:0006641 IGI:RGD P triglyceride metabolic process

KEGG Pathway Links

KEGG Pathway ID Description
ko04920 Adipocytokine signaling pathway
rno04920 Adipocytokine signaling pathway
ko00061 Fatty acid biosynthesis
rno00061 Fatty acid biosynthesis
ko00071 Fatty acid degradation
rno00071 Fatty acid degradation
ko01212 Fatty acid metabolism
rno01212 Fatty acid metabolism
rno01100 Metabolic pathways
ko03320 PPAR signaling pathway
rno03320 PPAR signaling pathway
ko04146 Peroxisome
rno04146 Peroxisome
M00086 beta-Oxidation, acyl-CoA synthesis

REACTOME Pathway Links

REACTOME Pathway ID Description
5953463 Fatty Acyl-CoA Biosynthesis
5953288 Fatty acid, triacylglycerol, and ketone body metabolism
5953250 Metabolism
5953289 Metabolism of lipids and lipoproteins
5953471 Synthesis of very long-chain fatty acyl-CoAs
5953464 Triglyceride Biosynthesis

Domain Information

InterPro Annotations

Accession Description
IPR020845 AMP-binding, conserved site
IPR000873 AMP-dependent synthetase/ligase

UniProt Annotations

Entry Information

Gene Name
acyl-CoA synthetase long-chain family member 6
Protein Entry
ACSL6_RAT
UniProt ID
P33124
Species
Rat

Comments

Comment Type Description
Alternative Products Event=Alternative splicing; Named isoforms=2; Name=1; Synonyms=ACSL6_vs1; IsoId=P33124-1; Sequence=Displayed; Note=Contains exon 14; Name=2; Synonyms=ACSL6_vs2; IsoId=P33124-2; Sequence=VSP_057107, VSP_057108; Note=Contains exon 13. No differences in the affinity for fatty acids and CoA. Decreased affinity for ATP. Could play an important role at lower ATP levels. Expressed at lower level in the brain compared to isoform 1. ;
Biophysicochemical Properties Kinetic parameters: KM=12210 uM for ATP (Isoform 1) ; KM=4.7 uM for CoA (Isoform 1) ; KM=6.0 uM for palmitate (Isoform 1) ; KM=3.0 uM for oleate (Isoform 1) ; KM=9.7 uM for arachidonate (Isoform 1) ; KM=1480 uM for ATP (Isoform 2) ; KM=2.4 uM for CoA (Isoform 2) ; KM=3.6 uM for palmitate (Isoform 2) ; KM=5.3 uM for oleate (Isoform 2) ; KM=6.5 uM for arachidonate (Isoform 2) ; Vmax=208 nmol/min/mg enzyme with palmitate as substrate (Isoform 1) ; Vmax=153 nmol/min/mg enzyme with oleate as substrate (Isoform 1) ; Vmax=139 nmol/min/mg enzyme with arachidonate as substrate (Isoform 1) ; Vmax=739 nmol/min/mg enzyme with palmitate as substrate (Isoform 2) ; Vmax=1088 nmol/min/mg enzyme with oleate as substrate (Isoform 2) ; Vmax=1212 nmol/min/mg enzyme with arachidonate as substrate (Isoform 2) ;
Catalytic Activity ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.
Cofactor Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
Developmental Stage Isoform 1 is detected 10 days after birth, and increased in a coordinate fashion during the development of brain, and the amount did not decrease when myelination was completed
Function Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Plays an important role in fatty acid metabolism in brain and the acyl-CoAs produced may be utilized exclusively for the synthesis of the brain lipid.
Miscellaneous 5 rat isozymes encoded by different genes have been described. ACSL6 corresponds to isozyme 2 (ACS2)
Similarity Belongs to the ATP-dependent AMP-binding enzyme family
Subcellular Location Mitochondrion outer membrane ; Single-pass type III membrane protein . Peroxisome membrane ; Single-pass type III membrane protein {ECO:0000250}. Microsome membrane ; Single-pass type III membrane protein . Endoplasmic reticulum membrane ; Single-pass type III membrane protein .
Tissue Specificity Expressed predominantly in brain and, to a much lesser extent, in heart and adrenal.

Identical and Related Proteins

Unique RefSeq proteins for LMP012893 (as displayed in Record Overview)

Protein GI Database Accession Length Protein Name
18543341 RefSeq NP_570095 697 long-chain-fatty-acid--CoA ligase 6

Identical Sequences to LMP012893 proteins

Reference Database Accession Length Protein Name
GI:18543341 GenBank AAB19809.2 697 phosphatidylinositol 4-kinase [Rattus sp.]

Related Sequences to LMP012893 proteins

Reference Database Accession Length Protein Name
GI:18543341 DBBJ BAA00932.1 697 long-chain acyl-CoA synthetase [Rattus norvegicus]
GI:18543341 GenBank AAB19809.2 697 phosphatidylinositol 4-kinase [Rattus sp.]
GI:18543341 SwissProt P33124.1 697 RecName: Full=Long-chain-fatty-acid--CoA ligase 6; AltName: Full=Long-chain acyl-CoA synthetase 6; Short=LACS 6; AltName: Full=Long-chain-fatty-acid--CoA ligase, brain isozyme [Rattus norvegicus]